Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH

Extrachromosomal DNA (ecDNA) is a common mode of oncogene amplification but is challenging to analyze. Here, we adapt CRISPR-CATCH, in vitro CRISPR-Cas9 treatment and pulsed field gel electrophoresis of agarose-entrapped genomic DNA, previously developed for bacterial chromosome segments, to isolate...

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Detalles Bibliográficos
Autores principales: Hung, King L., Luebeck, Jens, Dehkordi, Siavash R., Colón, Caterina I., Li, Rui, Wong, Ivy Tsz-Lo, Coruh, Ceyda, Dharanipragada, Prashanthi, Lomeli, Shirley H., Weiser, Natasha E., Moriceau, Gatien, Zhang, Xiao, Bailey, Chris, Houlahan, Kathleen E., Yang, Wenting, González, Rocío Chamorro, Swanton, Charles, Curtis, Christina, Jamal-Hanjani, Mariam, Henssen, Anton G., Law, Julie A., Greenleaf, William J., Lo, Roger S., Mischel, Paul S., Bafna, Vineet, Chang, Howard Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649439/
https://www.ncbi.nlm.nih.gov/pubmed/36253572
http://dx.doi.org/10.1038/s41588-022-01190-0

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