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Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples

As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for (13)C-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron Emission Tomography (PET), the technique s...

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Autor principal: Capozzi, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649762/
https://www.ncbi.nlm.nih.gov/pubmed/36357496
http://dx.doi.org/10.1038/s41598-022-23890-7
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author Capozzi, Andrea
author_facet Capozzi, Andrea
author_sort Capozzi, Andrea
collection PubMed
description As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for (13)C-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron Emission Tomography (PET), the technique struggles to enter everyday clinical practice. Because of the minute-long hyperpolarization lifetime after dissolution, one of the reasons lies in the need and consequent complexities of having the machine that generates the hyperpolarization (i.e. the dDNP polarizer) on site. Since some years, research groups are working to make hyperpolarization transportable. Two different methods have been developed that allow “freezing” of the nuclear spin state prior to samples extraction from the polarizer. Nevertheless, so far, all attempts of transport have been limited to a very small scale and to the level of proof-of-principle experiments. The main reason for that is the lack of adequate hardware, strategy, and control on most of the crucial parameters. To bridge the technical gap with PET and provide MRI facilities with hours long relaxing hyperpolarized compounds at controlled conditions, a new generation of low cost/small footprint liquid He cryostats equipped with a magnetically enforced cryogenic probe is needed. In this paper, we detail the theoretical and practical construction of a hyperpolarized samples transportation device small enough to fit in a car and able to hold a sample at 4.2 K for almost 8 h despite the presence of a cryogenically-demanding purpose-built probe that provides enough magnetic field upon insertion of the sample and NMR quality homogeneity at storage position. Should transportable hyperpolarization via DNP become a reality, we herein provide important details to make it possible.
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spelling pubmed-96497622022-11-15 Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples Capozzi, Andrea Sci Rep Article As of today, dissolution Dynamic Nuclear Polarization (dDNP) is the only clinically available hyperpolarization technique for (13)C-MRI. Despite the clear path towards personalized medicine that dDNP is paving as an alternative and/or complement to Positron Emission Tomography (PET), the technique struggles to enter everyday clinical practice. Because of the minute-long hyperpolarization lifetime after dissolution, one of the reasons lies in the need and consequent complexities of having the machine that generates the hyperpolarization (i.e. the dDNP polarizer) on site. Since some years, research groups are working to make hyperpolarization transportable. Two different methods have been developed that allow “freezing” of the nuclear spin state prior to samples extraction from the polarizer. Nevertheless, so far, all attempts of transport have been limited to a very small scale and to the level of proof-of-principle experiments. The main reason for that is the lack of adequate hardware, strategy, and control on most of the crucial parameters. To bridge the technical gap with PET and provide MRI facilities with hours long relaxing hyperpolarized compounds at controlled conditions, a new generation of low cost/small footprint liquid He cryostats equipped with a magnetically enforced cryogenic probe is needed. In this paper, we detail the theoretical and practical construction of a hyperpolarized samples transportation device small enough to fit in a car and able to hold a sample at 4.2 K for almost 8 h despite the presence of a cryogenically-demanding purpose-built probe that provides enough magnetic field upon insertion of the sample and NMR quality homogeneity at storage position. Should transportable hyperpolarization via DNP become a reality, we herein provide important details to make it possible. Nature Publishing Group UK 2022-11-10 /pmc/articles/PMC9649762/ /pubmed/36357496 http://dx.doi.org/10.1038/s41598-022-23890-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Capozzi, Andrea
Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title_full Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title_fullStr Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title_full_unstemmed Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title_short Design and performance of a small bath cryostat with NMR capability for transport of hyperpolarized samples
title_sort design and performance of a small bath cryostat with nmr capability for transport of hyperpolarized samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649762/
https://www.ncbi.nlm.nih.gov/pubmed/36357496
http://dx.doi.org/10.1038/s41598-022-23890-7
work_keys_str_mv AT capozziandrea designandperformanceofasmallbathcryostatwithnmrcapabilityfortransportofhyperpolarizedsamples