Cargando…

Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods

Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS). We found that...

Descripción completa

Detalles Bibliográficos
Autores principales: Alchera, Elisa, Monieri, Matteo, Maturi, Mirko, Locatelli, Irene, Locatelli, Erica, Tortorella, Silvia, Sacchi, Angelina, Corti, Angelo, Nebuloni, Manuela, Lucianò, Roberta, Pederzoli, Filippo, Montorsi, Francesco, Salonia, Andrea, Meyer, Sandra, Jose, Jithin, Giustetto, Pierangela, Franchini, Mauro Comes, Curnis, Flavio, Alfano, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649962/
https://www.ncbi.nlm.nih.gov/pubmed/36386292
http://dx.doi.org/10.1016/j.pacs.2022.100400
_version_ 1784827902350589952
author Alchera, Elisa
Monieri, Matteo
Maturi, Mirko
Locatelli, Irene
Locatelli, Erica
Tortorella, Silvia
Sacchi, Angelina
Corti, Angelo
Nebuloni, Manuela
Lucianò, Roberta
Pederzoli, Filippo
Montorsi, Francesco
Salonia, Andrea
Meyer, Sandra
Jose, Jithin
Giustetto, Pierangela
Franchini, Mauro Comes
Curnis, Flavio
Alfano, Massimo
author_facet Alchera, Elisa
Monieri, Matteo
Maturi, Mirko
Locatelli, Irene
Locatelli, Erica
Tortorella, Silvia
Sacchi, Angelina
Corti, Angelo
Nebuloni, Manuela
Lucianò, Roberta
Pederzoli, Filippo
Montorsi, Francesco
Salonia, Andrea
Meyer, Sandra
Jose, Jithin
Giustetto, Pierangela
Franchini, Mauro Comes
Curnis, Flavio
Alfano, Massimo
author_sort Alchera, Elisa
collection PubMed
description Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS). We found that the integrin α5β1, overexpressed in bladder cancer cell lines, murine orthotopic bladder cancer and human bladder CIS, can be exploited as a receptor for targeted delivery of GNRs functionalized with the cyclic CphgisoDGRG peptide (Iso4). The GNRs@Chit-Iso4 was stable in urine and selectively recognized α5β1 positive neoplastic urothelium, while low frequency ultrasound-assisted shaking of intravesically instilled GNRs@Chit-Iso4 allowed the distribution of nanoparticles across the entire volume of the bladder. Photoacoustic imaging of GNRs@Chit-Iso4 bound to tumor cells allowed for the detection of neoplastic lesions smaller than 0.5 mm that were undetectable by ultrasound imaging and bioluminescence.
format Online
Article
Text
id pubmed-9649962
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-96499622022-11-15 Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods Alchera, Elisa Monieri, Matteo Maturi, Mirko Locatelli, Irene Locatelli, Erica Tortorella, Silvia Sacchi, Angelina Corti, Angelo Nebuloni, Manuela Lucianò, Roberta Pederzoli, Filippo Montorsi, Francesco Salonia, Andrea Meyer, Sandra Jose, Jithin Giustetto, Pierangela Franchini, Mauro Comes Curnis, Flavio Alfano, Massimo Photoacoustics Editorial Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS). We found that the integrin α5β1, overexpressed in bladder cancer cell lines, murine orthotopic bladder cancer and human bladder CIS, can be exploited as a receptor for targeted delivery of GNRs functionalized with the cyclic CphgisoDGRG peptide (Iso4). The GNRs@Chit-Iso4 was stable in urine and selectively recognized α5β1 positive neoplastic urothelium, while low frequency ultrasound-assisted shaking of intravesically instilled GNRs@Chit-Iso4 allowed the distribution of nanoparticles across the entire volume of the bladder. Photoacoustic imaging of GNRs@Chit-Iso4 bound to tumor cells allowed for the detection of neoplastic lesions smaller than 0.5 mm that were undetectable by ultrasound imaging and bioluminescence. Elsevier 2022-08-30 /pmc/articles/PMC9649962/ /pubmed/36386292 http://dx.doi.org/10.1016/j.pacs.2022.100400 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Editorial
Alchera, Elisa
Monieri, Matteo
Maturi, Mirko
Locatelli, Irene
Locatelli, Erica
Tortorella, Silvia
Sacchi, Angelina
Corti, Angelo
Nebuloni, Manuela
Lucianò, Roberta
Pederzoli, Filippo
Montorsi, Francesco
Salonia, Andrea
Meyer, Sandra
Jose, Jithin
Giustetto, Pierangela
Franchini, Mauro Comes
Curnis, Flavio
Alfano, Massimo
Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title_full Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title_fullStr Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title_full_unstemmed Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title_short Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
title_sort early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
topic Editorial
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9649962/
https://www.ncbi.nlm.nih.gov/pubmed/36386292
http://dx.doi.org/10.1016/j.pacs.2022.100400
work_keys_str_mv AT alcheraelisa earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT monierimatteo earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT maturimirko earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT locatelliirene earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT locatellierica earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT tortorellasilvia earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT sacchiangelina earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT cortiangelo earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT nebulonimanuela earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT lucianoroberta earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT pederzolifilippo earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT montorsifrancesco earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT saloniaandrea earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT meyersandra earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT josejithin earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT giustettopierangela earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT franchinimaurocomes earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT curnisflavio earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods
AT alfanomassimo earlydiagnosisofbladdercancerbyphotoacousticimagingoftumortargetedgoldnanorods