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Protocol to evaluate hyperlipidemia in zebrafish larvae

In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utiliz...

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Detalles Bibliográficos
Autores principales: Balamurugan, Keerthana, Medishetti, Raghavender, Rao, Pallavi, K, Rahul Varma, Chatti, Kiranam, Parsa, Kishore V.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650076/
https://www.ncbi.nlm.nih.gov/pubmed/36386866
http://dx.doi.org/10.1016/j.xpro.2022.101819
Descripción
Sumario:In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly. For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022).