Protocol to evaluate hyperlipidemia in zebrafish larvae

In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utiliz...

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Autores principales: Balamurugan, Keerthana, Medishetti, Raghavender, Rao, Pallavi, K, Rahul Varma, Chatti, Kiranam, Parsa, Kishore V.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650076/
https://www.ncbi.nlm.nih.gov/pubmed/36386866
http://dx.doi.org/10.1016/j.xpro.2022.101819
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author Balamurugan, Keerthana
Medishetti, Raghavender
Rao, Pallavi
K, Rahul Varma
Chatti, Kiranam
Parsa, Kishore V.L.
author_facet Balamurugan, Keerthana
Medishetti, Raghavender
Rao, Pallavi
K, Rahul Varma
Chatti, Kiranam
Parsa, Kishore V.L.
author_sort Balamurugan, Keerthana
collection PubMed
description In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly. For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022).
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spelling pubmed-96500762022-11-15 Protocol to evaluate hyperlipidemia in zebrafish larvae Balamurugan, Keerthana Medishetti, Raghavender Rao, Pallavi K, Rahul Varma Chatti, Kiranam Parsa, Kishore V.L. STAR Protoc Protocol In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly. For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022). Elsevier 2022-11-07 /pmc/articles/PMC9650076/ /pubmed/36386866 http://dx.doi.org/10.1016/j.xpro.2022.101819 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Balamurugan, Keerthana
Medishetti, Raghavender
Rao, Pallavi
K, Rahul Varma
Chatti, Kiranam
Parsa, Kishore V.L.
Protocol to evaluate hyperlipidemia in zebrafish larvae
title Protocol to evaluate hyperlipidemia in zebrafish larvae
title_full Protocol to evaluate hyperlipidemia in zebrafish larvae
title_fullStr Protocol to evaluate hyperlipidemia in zebrafish larvae
title_full_unstemmed Protocol to evaluate hyperlipidemia in zebrafish larvae
title_short Protocol to evaluate hyperlipidemia in zebrafish larvae
title_sort protocol to evaluate hyperlipidemia in zebrafish larvae
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650076/
https://www.ncbi.nlm.nih.gov/pubmed/36386866
http://dx.doi.org/10.1016/j.xpro.2022.101819
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