Cargando…

Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial

The numbat (Myrmecobius fasciatus) is an endangered Australian marsupial, and the last surviving member of the Myrmecobiidae family. The numbat regularly undergoes torpor and is unique amongst marsupials as it is the only diurnal and termitivorous species. Here we sequenced the first draft genome of...

Descripción completa

Detalles Bibliográficos
Autores principales: Peel, Emma, Silver, Luke, Brandies, Parice, Hayakawa, Takashi, Belov, Katherine, Hogg, Carolyn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: GigaScience Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650251/
https://www.ncbi.nlm.nih.gov/pubmed/36824518
http://dx.doi.org/10.46471/gigabyte.47
_version_ 1784827971409805312
author Peel, Emma
Silver, Luke
Brandies, Parice
Hayakawa, Takashi
Belov, Katherine
Hogg, Carolyn J.
author_facet Peel, Emma
Silver, Luke
Brandies, Parice
Hayakawa, Takashi
Belov, Katherine
Hogg, Carolyn J.
author_sort Peel, Emma
collection PubMed
description The numbat (Myrmecobius fasciatus) is an endangered Australian marsupial, and the last surviving member of the Myrmecobiidae family. The numbat regularly undergoes torpor and is unique amongst marsupials as it is the only diurnal and termitivorous species. Here we sequenced the first draft genome of the numbat using 10× Genomics Chromium linked-read technology, resulting in a 3.42 Gbp genome with a scaffold N50 of 223 kbp. A global transcriptome from liver, lung and tongue was also generated to aid genome annotation, identifying 21,465 protein-coding genes. To investigate adaptation to the numbat’s termitivorous diet and arid/semi-arid range, we interrogated the most highly expressed transcripts within the tongue and manually annotated taste, vomeronasal and aquaporin gene families. Antimicrobial proteins and proteins involved in digestion were highly expressed in the tongue, alongside umami taste receptors. However, sweet taste receptors were not expressed in this tissue, which combined with the putative contraction of the bitter taste receptor gene repertoire in the numbat genome, may indicate a potential evolutionary adaptation to their specialised termitivorous diet. Vomeronasal and aquaporin gene repertoires were similar to other marsupials. The draft numbat genome is a valuable tool for conservation and can be applied to population genetics/genomics studies and to investigate the unique biology of this interesting species.
format Online
Article
Text
id pubmed-9650251
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher GigaScience Press
record_format MEDLINE/PubMed
spelling pubmed-96502512023-02-22 Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial Peel, Emma Silver, Luke Brandies, Parice Hayakawa, Takashi Belov, Katherine Hogg, Carolyn J. GigaByte Data Release The numbat (Myrmecobius fasciatus) is an endangered Australian marsupial, and the last surviving member of the Myrmecobiidae family. The numbat regularly undergoes torpor and is unique amongst marsupials as it is the only diurnal and termitivorous species. Here we sequenced the first draft genome of the numbat using 10× Genomics Chromium linked-read technology, resulting in a 3.42 Gbp genome with a scaffold N50 of 223 kbp. A global transcriptome from liver, lung and tongue was also generated to aid genome annotation, identifying 21,465 protein-coding genes. To investigate adaptation to the numbat’s termitivorous diet and arid/semi-arid range, we interrogated the most highly expressed transcripts within the tongue and manually annotated taste, vomeronasal and aquaporin gene families. Antimicrobial proteins and proteins involved in digestion were highly expressed in the tongue, alongside umami taste receptors. However, sweet taste receptors were not expressed in this tissue, which combined with the putative contraction of the bitter taste receptor gene repertoire in the numbat genome, may indicate a potential evolutionary adaptation to their specialised termitivorous diet. Vomeronasal and aquaporin gene repertoires were similar to other marsupials. The draft numbat genome is a valuable tool for conservation and can be applied to population genetics/genomics studies and to investigate the unique biology of this interesting species. GigaScience Press 2022-03-17 /pmc/articles/PMC9650251/ /pubmed/36824518 http://dx.doi.org/10.46471/gigabyte.47 Text en © The Author(s) 2022. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Release
Peel, Emma
Silver, Luke
Brandies, Parice
Hayakawa, Takashi
Belov, Katherine
Hogg, Carolyn J.
Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title_full Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title_fullStr Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title_full_unstemmed Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title_short Genome assembly of the numbat (Myrmecobius fasciatus), the only termitivorous marsupial
title_sort genome assembly of the numbat (myrmecobius fasciatus), the only termitivorous marsupial
topic Data Release
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650251/
https://www.ncbi.nlm.nih.gov/pubmed/36824518
http://dx.doi.org/10.46471/gigabyte.47
work_keys_str_mv AT peelemma genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial
AT silverluke genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial
AT brandiesparice genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial
AT hayakawatakashi genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial
AT belovkatherine genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial
AT hoggcarolynj genomeassemblyofthenumbatmyrmecobiusfasciatustheonlytermitivorousmarsupial