Cargando…
A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum)
Horsegram (Macrotyloma uniflorum [Lam.] Verdc.) is an underutilized warm-season diploid legume (2n = 20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a c...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
GigaScience Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650294/ https://www.ncbi.nlm.nih.gov/pubmed/36824333 http://dx.doi.org/10.46471/gigabyte.30 |
_version_ | 1784827982079066112 |
---|---|
author | Shirasawa, Kenta Chahota, Rakesh Hirakawa, Hideki Nagano, Soichiro Nagasaki, Hideki Sharma, Tilak Isobe, Sachiko |
author_facet | Shirasawa, Kenta Chahota, Rakesh Hirakawa, Hideki Nagano, Soichiro Nagasaki, Hideki Sharma, Tilak Isobe, Sachiko |
author_sort | Shirasawa, Kenta |
collection | PubMed |
description | Horsegram (Macrotyloma uniflorum [Lam.] Verdc.) is an underutilized warm-season diploid legume (2n = 20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for a horsegram variety, HPK-4. Ten chromosome-scale pseudomolecules were created by aligning Illumina scaffold sequences onto a linkage map. The total length of the ten pseudomolecules was 259.2 Mbp, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences. Diversity analysis of 89 horsegram accessions by dd-RAD-Seq identified 277 single nucleotide polymorphisms (SNPs), suggesting narrow genetic diversity among the horsegram accessions. This is the first attempt to generate a draft genome sequence of horsegram and will provide a reference for sequence-based analysis of horsegram germplasm. |
format | Online Article Text |
id | pubmed-9650294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | GigaScience Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-96502942023-02-22 A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) Shirasawa, Kenta Chahota, Rakesh Hirakawa, Hideki Nagano, Soichiro Nagasaki, Hideki Sharma, Tilak Isobe, Sachiko GigaByte Data Release Horsegram (Macrotyloma uniflorum [Lam.] Verdc.) is an underutilized warm-season diploid legume (2n = 20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for a horsegram variety, HPK-4. Ten chromosome-scale pseudomolecules were created by aligning Illumina scaffold sequences onto a linkage map. The total length of the ten pseudomolecules was 259.2 Mbp, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences. Diversity analysis of 89 horsegram accessions by dd-RAD-Seq identified 277 single nucleotide polymorphisms (SNPs), suggesting narrow genetic diversity among the horsegram accessions. This is the first attempt to generate a draft genome sequence of horsegram and will provide a reference for sequence-based analysis of horsegram germplasm. GigaScience Press 2021-10-08 /pmc/articles/PMC9650294/ /pubmed/36824333 http://dx.doi.org/10.46471/gigabyte.30 Text en © The Author(s) 2021. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Data Release Shirasawa, Kenta Chahota, Rakesh Hirakawa, Hideki Nagano, Soichiro Nagasaki, Hideki Sharma, Tilak Isobe, Sachiko A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title | A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title_full | A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title_fullStr | A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title_full_unstemmed | A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title_short | A chromosome-scale draft genome sequence of horsegram (Macrotyloma uniflorum) |
title_sort | chromosome-scale draft genome sequence of horsegram (macrotyloma uniflorum) |
topic | Data Release |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650294/ https://www.ncbi.nlm.nih.gov/pubmed/36824333 http://dx.doi.org/10.46471/gigabyte.30 |
work_keys_str_mv | AT shirasawakenta achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT chahotarakesh achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT hirakawahideki achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT naganosoichiro achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT nagasakihideki achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT sharmatilak achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT isobesachiko achromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT shirasawakenta chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT chahotarakesh chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT hirakawahideki chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT naganosoichiro chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT nagasakihideki chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT sharmatilak chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum AT isobesachiko chromosomescaledraftgenomesequenceofhorsegrammacrotylomauniflorum |