CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specif...

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Detalles Bibliográficos
Autores principales: Huang, Zhanchao, Fang, Jianhua, Zhou, Min, Gong, Zhenghua, Xiang, Tianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650447/
https://www.ncbi.nlm.nih.gov/pubmed/36386639
http://dx.doi.org/10.3389/fmicb.2022.1011399
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author Huang, Zhanchao
Fang, Jianhua
Zhou, Min
Gong, Zhenghua
Xiang, Tianxin
author_facet Huang, Zhanchao
Fang, Jianhua
Zhou, Min
Gong, Zhenghua
Xiang, Tianxin
author_sort Huang, Zhanchao
collection PubMed
description Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms. The review aims to provide insights to spur further development by summarizing the characteristics of effectors of the CRISPR-Cas13 system and by describing the latest research into its application in the rapid detection of pathogenic microorganisms in combination with nucleic acid extraction, isothermal amplification, and product detection.
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spelling pubmed-96504472022-11-15 CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms Huang, Zhanchao Fang, Jianhua Zhou, Min Gong, Zhenghua Xiang, Tianxin Front Microbiol Microbiology Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms. The review aims to provide insights to spur further development by summarizing the characteristics of effectors of the CRISPR-Cas13 system and by describing the latest research into its application in the rapid detection of pathogenic microorganisms in combination with nucleic acid extraction, isothermal amplification, and product detection. Frontiers Media S.A. 2022-10-28 /pmc/articles/PMC9650447/ /pubmed/36386639 http://dx.doi.org/10.3389/fmicb.2022.1011399 Text en Copyright © 2022 Huang, Fang, Zhou, Gong and Xiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Zhanchao
Fang, Jianhua
Zhou, Min
Gong, Zhenghua
Xiang, Tianxin
CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title_full CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title_fullStr CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title_full_unstemmed CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title_short CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
title_sort crispr-cas13: a new technology for the rapid detection of pathogenic microorganisms
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650447/
https://www.ncbi.nlm.nih.gov/pubmed/36386639
http://dx.doi.org/10.3389/fmicb.2022.1011399
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AT gongzhenghua crisprcas13anewtechnologyfortherapiddetectionofpathogenicmicroorganisms
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