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New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments

For more than 60 years, efforts to develop mating-based mosquito control technologies have largely failed to produce solutions that are both effective and scalable, keeping them out of reach of most governments and communities in disease-impacted regions globally. High pest suppression levels in tri...

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Autores principales: Spinner, Siân A. M., Barnes, Zoe H., Puinean, Alin Mirel, Gray, Pam, Dafa’alla, Tarig, Phillips, Caroline E., Nascimento de Souza, Camila, Frazon, Tamires Fonseca, Ercit, Kyla, Collado, Amandine, Naish, Neil, Sulston, Edward, Ll. Phillips, Gwilym C., Greene, Kelleigh K., Poletto, Mattia, Sperry, Benjamin D., Warner, Simon A., Rose, Nathan R., Frandsen, Grey K., Verza, Natalia C., Gorman, Kevin J., Matzen, Kelly J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650594/
https://www.ncbi.nlm.nih.gov/pubmed/36394032
http://dx.doi.org/10.3389/fbioe.2022.975786
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author Spinner, Siân A. M.
Barnes, Zoe H.
Puinean, Alin Mirel
Gray, Pam
Dafa’alla, Tarig
Phillips, Caroline E.
Nascimento de Souza, Camila
Frazon, Tamires Fonseca
Ercit, Kyla
Collado, Amandine
Naish, Neil
Sulston, Edward
Ll. Phillips, Gwilym C.
Greene, Kelleigh K.
Poletto, Mattia
Sperry, Benjamin D.
Warner, Simon A.
Rose, Nathan R.
Frandsen, Grey K.
Verza, Natalia C.
Gorman, Kevin J.
Matzen, Kelly J.
author_facet Spinner, Siân A. M.
Barnes, Zoe H.
Puinean, Alin Mirel
Gray, Pam
Dafa’alla, Tarig
Phillips, Caroline E.
Nascimento de Souza, Camila
Frazon, Tamires Fonseca
Ercit, Kyla
Collado, Amandine
Naish, Neil
Sulston, Edward
Ll. Phillips, Gwilym C.
Greene, Kelleigh K.
Poletto, Mattia
Sperry, Benjamin D.
Warner, Simon A.
Rose, Nathan R.
Frandsen, Grey K.
Verza, Natalia C.
Gorman, Kevin J.
Matzen, Kelly J.
author_sort Spinner, Siân A. M.
collection PubMed
description For more than 60 years, efforts to develop mating-based mosquito control technologies have largely failed to produce solutions that are both effective and scalable, keeping them out of reach of most governments and communities in disease-impacted regions globally. High pest suppression levels in trials have yet to fully translate into broad and effective Aedes aegypti control solutions. Two primary challenges to date–the need for complex sex-sorting to prevent female releases, and cumbersome processes for rearing and releasing male adult mosquitoes–present significant barriers for existing methods. As the host range of Aedes aegypti continues to advance into new geographies due to increasing globalisation and climate change, traditional chemical-based approaches are under mounting pressure from both more stringent regulatory processes and the ongoing development of insecticide resistance. It is no exaggeration to state that new tools, which are equal parts effective and scalable, are needed now more than ever. This paper describes the development and field evaluation of a new self-sexing strain of Aedes aegypti that has been designed to combine targeted vector suppression, operational simplicity, and cost-effectiveness for use in disease-prone regions. This conditional, self-limiting trait uses the sex-determination gene doublesex linked to the tetracycline-off genetic switch to cause complete female lethality in early larval development. With no female progeny survival, sex sorting is no longer required, eliminating the need for large-scale mosquito production facilities or physical sex-separation. In deployment operations, this translates to the ability to generate multiple generations of suppression for each mosquito released, while being entirely self-limiting. To evaluate these potential benefits, a field trial was carried out in densely-populated urban, dengue-prone neighbourhoods in Brazil, wherein the strain was able to suppress wild mosquito populations by up to 96%, demonstrating the utility of this self-sexing approach for biological vector control. In doing so, it has shown that such strains offer the critical components necessary to make these tools highly accessible, and thus they harbour the potential to transition mating-based approaches to effective and sustainable vector control tools that are within reach of governments and at-risk communities who may have only limited resources.
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spelling pubmed-96505942022-11-15 New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments Spinner, Siân A. M. Barnes, Zoe H. Puinean, Alin Mirel Gray, Pam Dafa’alla, Tarig Phillips, Caroline E. Nascimento de Souza, Camila Frazon, Tamires Fonseca Ercit, Kyla Collado, Amandine Naish, Neil Sulston, Edward Ll. Phillips, Gwilym C. Greene, Kelleigh K. Poletto, Mattia Sperry, Benjamin D. Warner, Simon A. Rose, Nathan R. Frandsen, Grey K. Verza, Natalia C. Gorman, Kevin J. Matzen, Kelly J. Front Bioeng Biotechnol Bioengineering and Biotechnology For more than 60 years, efforts to develop mating-based mosquito control technologies have largely failed to produce solutions that are both effective and scalable, keeping them out of reach of most governments and communities in disease-impacted regions globally. High pest suppression levels in trials have yet to fully translate into broad and effective Aedes aegypti control solutions. Two primary challenges to date–the need for complex sex-sorting to prevent female releases, and cumbersome processes for rearing and releasing male adult mosquitoes–present significant barriers for existing methods. As the host range of Aedes aegypti continues to advance into new geographies due to increasing globalisation and climate change, traditional chemical-based approaches are under mounting pressure from both more stringent regulatory processes and the ongoing development of insecticide resistance. It is no exaggeration to state that new tools, which are equal parts effective and scalable, are needed now more than ever. This paper describes the development and field evaluation of a new self-sexing strain of Aedes aegypti that has been designed to combine targeted vector suppression, operational simplicity, and cost-effectiveness for use in disease-prone regions. This conditional, self-limiting trait uses the sex-determination gene doublesex linked to the tetracycline-off genetic switch to cause complete female lethality in early larval development. With no female progeny survival, sex sorting is no longer required, eliminating the need for large-scale mosquito production facilities or physical sex-separation. In deployment operations, this translates to the ability to generate multiple generations of suppression for each mosquito released, while being entirely self-limiting. To evaluate these potential benefits, a field trial was carried out in densely-populated urban, dengue-prone neighbourhoods in Brazil, wherein the strain was able to suppress wild mosquito populations by up to 96%, demonstrating the utility of this self-sexing approach for biological vector control. In doing so, it has shown that such strains offer the critical components necessary to make these tools highly accessible, and thus they harbour the potential to transition mating-based approaches to effective and sustainable vector control tools that are within reach of governments and at-risk communities who may have only limited resources. Frontiers Media S.A. 2022-10-25 /pmc/articles/PMC9650594/ /pubmed/36394032 http://dx.doi.org/10.3389/fbioe.2022.975786 Text en Copyright © 2022 Spinner, Barnes, Puinean, Gray, Dafa’alla, Phillips, Nascimento de Souza, Frazon, Ercit, Collado, Naish, Sulston, Ll. Phillips, Greene, Poletto, Sperry, Warner, Rose, Frandsen, Verza, Gorman and Matzen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Spinner, Siân A. M.
Barnes, Zoe H.
Puinean, Alin Mirel
Gray, Pam
Dafa’alla, Tarig
Phillips, Caroline E.
Nascimento de Souza, Camila
Frazon, Tamires Fonseca
Ercit, Kyla
Collado, Amandine
Naish, Neil
Sulston, Edward
Ll. Phillips, Gwilym C.
Greene, Kelleigh K.
Poletto, Mattia
Sperry, Benjamin D.
Warner, Simon A.
Rose, Nathan R.
Frandsen, Grey K.
Verza, Natalia C.
Gorman, Kevin J.
Matzen, Kelly J.
New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title_full New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title_fullStr New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title_full_unstemmed New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title_short New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
title_sort new self-sexing aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650594/
https://www.ncbi.nlm.nih.gov/pubmed/36394032
http://dx.doi.org/10.3389/fbioe.2022.975786
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