Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022

Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tes...

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Autores principales: Michel, Janine, Targosz, Angelina, Rinner, Thomas, Bourquain, Daniel, Brinkmann, Annika, Sacks, Jilian Amber, Schaade, Lars, Nitsche, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2022
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650706/
https://www.ncbi.nlm.nih.gov/pubmed/36367010
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.45.2200816
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author Michel, Janine
Targosz, Angelina
Rinner, Thomas
Bourquain, Daniel
Brinkmann, Annika
Sacks, Jilian Amber
Schaade, Lars
Nitsche, Andreas
author_facet Michel, Janine
Targosz, Angelina
Rinner, Thomas
Bourquain, Daniel
Brinkmann, Annika
Sacks, Jilian Amber
Schaade, Lars
Nitsche, Andreas
author_sort Michel, Janine
collection PubMed
description Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tested kits are currently intended for research use only and clinical performance still needs to be assessed in more detail, but all were suitable for diagnostics of monkeypox virus, with variations in specificity rather than sensitivity.
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spelling pubmed-96507062022-11-16 Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022 Michel, Janine Targosz, Angelina Rinner, Thomas Bourquain, Daniel Brinkmann, Annika Sacks, Jilian Amber Schaade, Lars Nitsche, Andreas Euro Surveill Rapid Communication Before the international spread of monkeypox in May 2022, PCR kits for the detection of orthopoxviruses, and specifically monkeypox virus, were rarely available. Here we describe the evaluation of 11 recently developed commercially available PCR kits for the detection of monkeypox virus DNA. All tested kits are currently intended for research use only and clinical performance still needs to be assessed in more detail, but all were suitable for diagnostics of monkeypox virus, with variations in specificity rather than sensitivity. European Centre for Disease Prevention and Control (ECDC) 2022-11-10 /pmc/articles/PMC9650706/ /pubmed/36367010 http://dx.doi.org/10.2807/1560-7917.ES.2022.27.45.2200816 Text en This article is copyright of the authors or their affiliated institutions, 2022. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Michel, Janine
Targosz, Angelina
Rinner, Thomas
Bourquain, Daniel
Brinkmann, Annika
Sacks, Jilian Amber
Schaade, Lars
Nitsche, Andreas
Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title_full Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title_fullStr Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title_full_unstemmed Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title_short Evaluation of 11 commercially available PCR kits for the detection of monkeypox virus DNA, Berlin, July to September 2022
title_sort evaluation of 11 commercially available pcr kits for the detection of monkeypox virus dna, berlin, july to september 2022
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650706/
https://www.ncbi.nlm.nih.gov/pubmed/36367010
http://dx.doi.org/10.2807/1560-7917.ES.2022.27.45.2200816
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