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Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva

How larvae of the many phyla of marine invertebrates find places appropriate for settlement, metamorphosis, growth, and reproduction is an enduring question in marine science. Biofilm-induced metamorphosis has been observed in marine invertebrate larvae from nearly every major marine phylum. Despite...

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Autores principales: Freckelton, Marnie L., Nedved, Brian T., Cai, You-Sheng, Cao, Shugeng, Turano, Helen, Alegado, Rosanna A., Hadfield, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9651628/
https://www.ncbi.nlm.nih.gov/pubmed/35467986
http://dx.doi.org/10.1073/pnas.2200795119
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author Freckelton, Marnie L.
Nedved, Brian T.
Cai, You-Sheng
Cao, Shugeng
Turano, Helen
Alegado, Rosanna A.
Hadfield, Michael G.
author_facet Freckelton, Marnie L.
Nedved, Brian T.
Cai, You-Sheng
Cao, Shugeng
Turano, Helen
Alegado, Rosanna A.
Hadfield, Michael G.
author_sort Freckelton, Marnie L.
collection PubMed
description How larvae of the many phyla of marine invertebrates find places appropriate for settlement, metamorphosis, growth, and reproduction is an enduring question in marine science. Biofilm-induced metamorphosis has been observed in marine invertebrate larvae from nearly every major marine phylum. Despite the widespread nature of this phenomenon, the mechanism of induction remains poorly understood. The serpulid polychaete Hydroides elegans is a well established model for investigating bacteria-induced larval development. A broad range of biofilm bacterial species elicit larval metamorphosis in H. elegans via at least two mechanisms, including outer membrane vesicles (OMVs) and complexes of phage-tail bacteriocins. We investigated the interaction between larvae of H. elegans and the inductive bacterium Cellulophaga lytica, which produces an abundance of OMVs but not phage-tail bacteriocins. We asked whether the OMVs of C. lytica induce larval settlement due to cell membrane components or through delivery of specific cargo. Employing a biochemical structure–function approach with a strong ecological focus, the cells and OMVs produced by C. lytica were interrogated to determine the class of the inductive compounds. Here, we report that larvae of H. elegans are induced to metamorphose by lipopolysaccharide produced by C. lytica. The widespread prevalence of lipopolysaccharide and its associated taxonomic and structural variability suggest it may be a broadly employed cue for bacterially induced larval settlement of marine invertebrates.
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spelling pubmed-96516282022-11-15 Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva Freckelton, Marnie L. Nedved, Brian T. Cai, You-Sheng Cao, Shugeng Turano, Helen Alegado, Rosanna A. Hadfield, Michael G. Proc Natl Acad Sci U S A Biological Sciences How larvae of the many phyla of marine invertebrates find places appropriate for settlement, metamorphosis, growth, and reproduction is an enduring question in marine science. Biofilm-induced metamorphosis has been observed in marine invertebrate larvae from nearly every major marine phylum. Despite the widespread nature of this phenomenon, the mechanism of induction remains poorly understood. The serpulid polychaete Hydroides elegans is a well established model for investigating bacteria-induced larval development. A broad range of biofilm bacterial species elicit larval metamorphosis in H. elegans via at least two mechanisms, including outer membrane vesicles (OMVs) and complexes of phage-tail bacteriocins. We investigated the interaction between larvae of H. elegans and the inductive bacterium Cellulophaga lytica, which produces an abundance of OMVs but not phage-tail bacteriocins. We asked whether the OMVs of C. lytica induce larval settlement due to cell membrane components or through delivery of specific cargo. Employing a biochemical structure–function approach with a strong ecological focus, the cells and OMVs produced by C. lytica were interrogated to determine the class of the inductive compounds. Here, we report that larvae of H. elegans are induced to metamorphose by lipopolysaccharide produced by C. lytica. The widespread prevalence of lipopolysaccharide and its associated taxonomic and structural variability suggest it may be a broadly employed cue for bacterially induced larval settlement of marine invertebrates. National Academy of Sciences 2022-04-25 2022-05-03 /pmc/articles/PMC9651628/ /pubmed/35467986 http://dx.doi.org/10.1073/pnas.2200795119 Text en Copyright © 2022 the Author(s). Published by PNAS https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Freckelton, Marnie L.
Nedved, Brian T.
Cai, You-Sheng
Cao, Shugeng
Turano, Helen
Alegado, Rosanna A.
Hadfield, Michael G.
Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title_full Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title_fullStr Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title_full_unstemmed Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title_short Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
title_sort bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9651628/
https://www.ncbi.nlm.nih.gov/pubmed/35467986
http://dx.doi.org/10.1073/pnas.2200795119
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