Seasonal accumulation of photoassimilated carbon relates to growth rate and use for new aboveground organs of young apple trees in following spring

Deciduous trees accumulate carbon (C) in woody parts during the growth season which is subsequently used for the initial development and growth of newly formed organs in the following season; however, it is unclear which period during the growth season contributes to C accumulation. Three-year-old potted Malus domestica (apple) trees were grown in controlled growth chambers during the growth season and exposed to (13)CO(2) in an exposure chamber at seven different periods of the growth season, including vegetative and reproductive growth periods. Approximately half of the trees were harvested in late autumn, and the remaining trees were grown in a field in the following year. The (13)C accumulation in the different organs in late autumn, and its concentration in the new aboveground growth during the following growth season, was determined. The concentration of the photoassimilated (13)C in woody parts (shoots, trunk, rootstock and coarse roots) in the late autumn was higher in the trees labeled during the period of vigorous vegetative growth than in those labeled during other periods of growth. Furthermore, (13)C concentration in the leaves, annual shoots, flower buds and flowers in the following early spring was also high in the trees labeled during this period. The concentration of (13)C in the flower buds and flowers was positively correlated with that in the woody parts in the late autumn and old shoots in the following spring. Hence, the seasonal accumulation of photoassimilated C in woody parts in late autumn is related to growth rates during the growth season and its use for the initial development of newly formed organs in the following spring. These results suggest that under non-stressed conditions, C accumulated during the period of vigorous vegetative growth largely contributes to the C reserves that are used for the development of new organs in the following year.