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An electrochemical biosensor for the rapid genetic identification of Musang King durian

More than 200 different cultivars of durian exist worldwide but Durio zibethinus or Musang King (MK) is the most premium and prized durian fruit among the recommended varieties. Early identification of this premium variety is critical to protect from non-authentic MK durian cultivars. However, the M...

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Autores principales: Azizi, Mohammad Malek Faizal, Romeli, Sohana, Razali, Hazana, Ariffin, Eda Yuhana, Tajol Ariffin, Muhammad Afiq, Heng, Lee Yook, Abu-Bakar, Norliza, Lau, Han Yih
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652400/
https://www.ncbi.nlm.nih.gov/pubmed/36369187
http://dx.doi.org/10.1038/s41598-022-20998-8
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author Azizi, Mohammad Malek Faizal
Romeli, Sohana
Razali, Hazana
Ariffin, Eda Yuhana
Tajol Ariffin, Muhammad Afiq
Heng, Lee Yook
Abu-Bakar, Norliza
Lau, Han Yih
author_facet Azizi, Mohammad Malek Faizal
Romeli, Sohana
Razali, Hazana
Ariffin, Eda Yuhana
Tajol Ariffin, Muhammad Afiq
Heng, Lee Yook
Abu-Bakar, Norliza
Lau, Han Yih
author_sort Azizi, Mohammad Malek Faizal
collection PubMed
description More than 200 different cultivars of durian exist worldwide but Durio zibethinus or Musang King (MK) is the most premium and prized durian fruit among the recommended varieties. Early identification of this premium variety is critical to protect from non-authentic MK durian cultivars. However, the MK variety's morphological traits are nearly identical to other varieties. Currently, the identification of durian varieties is mostly performed via evaluation of leaf shape, fruit shape, aroma, taste and seed shape and this requires trained personnel for the morphology observation. To enable the rapid identification of the MK variety, PCR amplification of ten durian varieties using six gene candidates from the chloroplast genome was first performed to obtain DNA probes that were specific to the MK durian variety. PCR amplification of ten durian varieties using primers designed confirmed that the nadhA gene sequence showed an obvious difference in the MK variety from other durian varieties. The unique sequence of MK was used as a DNA probe to develop an electrochemical biosensor for the direct identification of the MK durian variety. The electrochemical biosensor was based on the hybridization response of the immobilized DNA probe with the target DNA from the MK variety and was monitored via differential pulse voltammetry technique. Under optimal conditions, the DNA electrochemical biosensor showed a low detection limit at 10% of MK genomic DNA concentration with a wide linear calibration range of 0.05–1.5 µM (R(2) = 0.9891) and RSD value of 3.77% (n = 3). The results of the developed DNA biosensor provide high promise for the development of portable sensors employed in the determination of MK variety in the field.
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spelling pubmed-96524002022-11-15 An electrochemical biosensor for the rapid genetic identification of Musang King durian Azizi, Mohammad Malek Faizal Romeli, Sohana Razali, Hazana Ariffin, Eda Yuhana Tajol Ariffin, Muhammad Afiq Heng, Lee Yook Abu-Bakar, Norliza Lau, Han Yih Sci Rep Article More than 200 different cultivars of durian exist worldwide but Durio zibethinus or Musang King (MK) is the most premium and prized durian fruit among the recommended varieties. Early identification of this premium variety is critical to protect from non-authentic MK durian cultivars. However, the MK variety's morphological traits are nearly identical to other varieties. Currently, the identification of durian varieties is mostly performed via evaluation of leaf shape, fruit shape, aroma, taste and seed shape and this requires trained personnel for the morphology observation. To enable the rapid identification of the MK variety, PCR amplification of ten durian varieties using six gene candidates from the chloroplast genome was first performed to obtain DNA probes that were specific to the MK durian variety. PCR amplification of ten durian varieties using primers designed confirmed that the nadhA gene sequence showed an obvious difference in the MK variety from other durian varieties. The unique sequence of MK was used as a DNA probe to develop an electrochemical biosensor for the direct identification of the MK durian variety. The electrochemical biosensor was based on the hybridization response of the immobilized DNA probe with the target DNA from the MK variety and was monitored via differential pulse voltammetry technique. Under optimal conditions, the DNA electrochemical biosensor showed a low detection limit at 10% of MK genomic DNA concentration with a wide linear calibration range of 0.05–1.5 µM (R(2) = 0.9891) and RSD value of 3.77% (n = 3). The results of the developed DNA biosensor provide high promise for the development of portable sensors employed in the determination of MK variety in the field. Nature Publishing Group UK 2022-11-11 /pmc/articles/PMC9652400/ /pubmed/36369187 http://dx.doi.org/10.1038/s41598-022-20998-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Azizi, Mohammad Malek Faizal
Romeli, Sohana
Razali, Hazana
Ariffin, Eda Yuhana
Tajol Ariffin, Muhammad Afiq
Heng, Lee Yook
Abu-Bakar, Norliza
Lau, Han Yih
An electrochemical biosensor for the rapid genetic identification of Musang King durian
title An electrochemical biosensor for the rapid genetic identification of Musang King durian
title_full An electrochemical biosensor for the rapid genetic identification of Musang King durian
title_fullStr An electrochemical biosensor for the rapid genetic identification of Musang King durian
title_full_unstemmed An electrochemical biosensor for the rapid genetic identification of Musang King durian
title_short An electrochemical biosensor for the rapid genetic identification of Musang King durian
title_sort electrochemical biosensor for the rapid genetic identification of musang king durian
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652400/
https://www.ncbi.nlm.nih.gov/pubmed/36369187
http://dx.doi.org/10.1038/s41598-022-20998-8
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