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Mesenchymal stem cells alleviate aging in vitro and in vivo

BACKGROUND: Aging is a natural and multi-factorial phenomenon associated with multiple human pathologies. Mesenchymal stem cells (MSCs) hold great promise in clinical fields of medicine including tissue repair, cardiovascular disease, and brain ischemic injury. The purpose of this study was to explo...

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Autores principales: Liu, Qun, Song, Shaole, Song, Lei, Bi, Youkun, Zhu, Keqi, Qiao, Xinlong, Wang, Huiwen, Gao, Chao, Cai, Hong, Ji, Guangju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652517/
https://www.ncbi.nlm.nih.gov/pubmed/36388801
http://dx.doi.org/10.21037/atm-22-1206
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author Liu, Qun
Song, Shaole
Song, Lei
Bi, Youkun
Zhu, Keqi
Qiao, Xinlong
Wang, Huiwen
Gao, Chao
Cai, Hong
Ji, Guangju
author_facet Liu, Qun
Song, Shaole
Song, Lei
Bi, Youkun
Zhu, Keqi
Qiao, Xinlong
Wang, Huiwen
Gao, Chao
Cai, Hong
Ji, Guangju
author_sort Liu, Qun
collection PubMed
description BACKGROUND: Aging is a natural and multi-factorial phenomenon associated with multiple human pathologies. Mesenchymal stem cells (MSCs) hold great promise in clinical fields of medicine including tissue repair, cardiovascular disease, and brain ischemic injury. The purpose of this study was to explore the roles of MSCs in improving the condition of aging cells, repairing aging tissues and organs, and extending the life span of elderly mice. METHODS: This study was carried out both in vitro and in vivo. We used MSCs to intervene with IMR-90 senescent cells induced by D-galactose and aged C57BL/6 mice. RESULTS: After 48 hours of co-culturing the aged cells with MSCs, the up-regulated expression of inflammatory factor, interleukin 6 (IL6), and the down-regulated expression of several growth factors, such as transforming growth factor (TGFβ1) and growth differentiation factor (GDF11), in D-galactose induced senescent cells were reversed. Moreover, compared with aged cells, the number of mitochondria and the telomere length were increased with MSC treatment. Similarly, in aged mice, the symptoms related to aging were improved after MSC treatment: the mouse hair became shiny and dense, and the symptoms of bladder overactivity were relieved. Hematoxylin and eosin (H&E) and Masson’s trichrome staining showed that the histopathological changes in skin, bladder, liver, and lung were apparently improved. CONCLUSIONS: Treatment with MSCs effectively improves aging-related phenotypes and plays a beneficial role in improving aging and aging-related diseases.
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spelling pubmed-96525172022-11-15 Mesenchymal stem cells alleviate aging in vitro and in vivo Liu, Qun Song, Shaole Song, Lei Bi, Youkun Zhu, Keqi Qiao, Xinlong Wang, Huiwen Gao, Chao Cai, Hong Ji, Guangju Ann Transl Med Original Article BACKGROUND: Aging is a natural and multi-factorial phenomenon associated with multiple human pathologies. Mesenchymal stem cells (MSCs) hold great promise in clinical fields of medicine including tissue repair, cardiovascular disease, and brain ischemic injury. The purpose of this study was to explore the roles of MSCs in improving the condition of aging cells, repairing aging tissues and organs, and extending the life span of elderly mice. METHODS: This study was carried out both in vitro and in vivo. We used MSCs to intervene with IMR-90 senescent cells induced by D-galactose and aged C57BL/6 mice. RESULTS: After 48 hours of co-culturing the aged cells with MSCs, the up-regulated expression of inflammatory factor, interleukin 6 (IL6), and the down-regulated expression of several growth factors, such as transforming growth factor (TGFβ1) and growth differentiation factor (GDF11), in D-galactose induced senescent cells were reversed. Moreover, compared with aged cells, the number of mitochondria and the telomere length were increased with MSC treatment. Similarly, in aged mice, the symptoms related to aging were improved after MSC treatment: the mouse hair became shiny and dense, and the symptoms of bladder overactivity were relieved. Hematoxylin and eosin (H&E) and Masson’s trichrome staining showed that the histopathological changes in skin, bladder, liver, and lung were apparently improved. CONCLUSIONS: Treatment with MSCs effectively improves aging-related phenotypes and plays a beneficial role in improving aging and aging-related diseases. AME Publishing Company 2022-10 /pmc/articles/PMC9652517/ /pubmed/36388801 http://dx.doi.org/10.21037/atm-22-1206 Text en 2022 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Liu, Qun
Song, Shaole
Song, Lei
Bi, Youkun
Zhu, Keqi
Qiao, Xinlong
Wang, Huiwen
Gao, Chao
Cai, Hong
Ji, Guangju
Mesenchymal stem cells alleviate aging in vitro and in vivo
title Mesenchymal stem cells alleviate aging in vitro and in vivo
title_full Mesenchymal stem cells alleviate aging in vitro and in vivo
title_fullStr Mesenchymal stem cells alleviate aging in vitro and in vivo
title_full_unstemmed Mesenchymal stem cells alleviate aging in vitro and in vivo
title_short Mesenchymal stem cells alleviate aging in vitro and in vivo
title_sort mesenchymal stem cells alleviate aging in vitro and in vivo
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652517/
https://www.ncbi.nlm.nih.gov/pubmed/36388801
http://dx.doi.org/10.21037/atm-22-1206
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