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The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines

BACKGROUND: Pancreatic cancer does not show any symptoms in the early period and metastatic process is already passed when the diagnosis is done. Therefore, in the battle with pancreatic cancer, novel treatment strategies, particularly antiinvasive and antimetastatic strategies, are needed. The cyto...

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Autores principales: Arikoglu, Hilal, Dursunoglu, Duygu, Kaya, Dudu Erkoc, Avci, Ebru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Makerere Medical School 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652687/
https://www.ncbi.nlm.nih.gov/pubmed/36407358
http://dx.doi.org/10.4314/ahs.v22i2.37
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author Arikoglu, Hilal
Dursunoglu, Duygu
Kaya, Dudu Erkoc
Avci, Ebru
author_facet Arikoglu, Hilal
Dursunoglu, Duygu
Kaya, Dudu Erkoc
Avci, Ebru
author_sort Arikoglu, Hilal
collection PubMed
description BACKGROUND: Pancreatic cancer does not show any symptoms in the early period and metastatic process is already passed when the diagnosis is done. Therefore, in the battle with pancreatic cancer, novel treatment strategies, particularly antiinvasive and antimetastatic strategies, are needed. The cytotoxic and anticancer effects of juglone and sodium selenite (NaSe) have been showed in various cancer cells. OBJECTIVES: In this study, it is aimed to investigate the synergistic effects of juglone and selenium on PANC-1 and BxPC-3 pancreatic cancer cells. METHODS: Antimetastatic effects of juglone-NaSe were carried out by adhesion and invasion assays and the genes and protein expressions. Expression analysis of the CDH1, ITGB3 and COL4A3 genes and their proteins E-cadherin, β3 integrin and tumstatin which play role in metastasis and angiogenesis processes, were done by qPCR and immunohistochemical analysis, respectively. RESULTS: Study findings have provided evidences that the juglone-selenium has a cytotoxic and dose dependent suppressive effect on invasion and metastasis in PANC-1 and BxPC-3 cells. CONCLUSION: The juglone-NaSe has the potential to be a promising agent especially to inhibit invasion and metastasis in pancreatic cancer treatment. However, more in depth studies are needed to more clearly demonstrate the effects of juglone-selenium.
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spelling pubmed-96526872022-11-18 The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines Arikoglu, Hilal Dursunoglu, Duygu Kaya, Dudu Erkoc Avci, Ebru Afr Health Sci Articles BACKGROUND: Pancreatic cancer does not show any symptoms in the early period and metastatic process is already passed when the diagnosis is done. Therefore, in the battle with pancreatic cancer, novel treatment strategies, particularly antiinvasive and antimetastatic strategies, are needed. The cytotoxic and anticancer effects of juglone and sodium selenite (NaSe) have been showed in various cancer cells. OBJECTIVES: In this study, it is aimed to investigate the synergistic effects of juglone and selenium on PANC-1 and BxPC-3 pancreatic cancer cells. METHODS: Antimetastatic effects of juglone-NaSe were carried out by adhesion and invasion assays and the genes and protein expressions. Expression analysis of the CDH1, ITGB3 and COL4A3 genes and their proteins E-cadherin, β3 integrin and tumstatin which play role in metastasis and angiogenesis processes, were done by qPCR and immunohistochemical analysis, respectively. RESULTS: Study findings have provided evidences that the juglone-selenium has a cytotoxic and dose dependent suppressive effect on invasion and metastasis in PANC-1 and BxPC-3 cells. CONCLUSION: The juglone-NaSe has the potential to be a promising agent especially to inhibit invasion and metastasis in pancreatic cancer treatment. However, more in depth studies are needed to more clearly demonstrate the effects of juglone-selenium. Makerere Medical School 2022-06 /pmc/articles/PMC9652687/ /pubmed/36407358 http://dx.doi.org/10.4314/ahs.v22i2.37 Text en © 2022 Arikoglu H et al. https://creativecommons.org/licenses/by/4.0/Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Arikoglu, Hilal
Dursunoglu, Duygu
Kaya, Dudu Erkoc
Avci, Ebru
The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title_full The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title_fullStr The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title_full_unstemmed The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title_short The effects of Juglone-Selenium combination on invasion and metastasis in pancreatic cancer cell lines
title_sort effects of juglone-selenium combination on invasion and metastasis in pancreatic cancer cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652687/
https://www.ncbi.nlm.nih.gov/pubmed/36407358
http://dx.doi.org/10.4314/ahs.v22i2.37
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