Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system

Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visual...

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Detalles Bibliográficos
Autores principales: Fujisawa, Yuki, Ming, Lu, Yamada, Daisuke, Takao, Tomoka, Takarada, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652995/
https://www.ncbi.nlm.nih.gov/pubmed/36371298
http://dx.doi.org/10.1186/s13287-022-03203-5
Descripción
Sumario:Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX(+) cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato(+) cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato(+) cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-022-03203-5.

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