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Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system
Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visual...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652995/ https://www.ncbi.nlm.nih.gov/pubmed/36371298 http://dx.doi.org/10.1186/s13287-022-03203-5 |
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author | Fujisawa, Yuki Ming, Lu Yamada, Daisuke Takao, Tomoka Takarada, Takeshi |
author_facet | Fujisawa, Yuki Ming, Lu Yamada, Daisuke Takao, Tomoka Takarada, Takeshi |
author_sort | Fujisawa, Yuki |
collection | PubMed |
description | Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX(+) cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato(+) cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato(+) cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-022-03203-5. |
format | Online Article Text |
id | pubmed-9652995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-96529952022-11-15 Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system Fujisawa, Yuki Ming, Lu Yamada, Daisuke Takao, Tomoka Takarada, Takeshi Stem Cell Res Ther Short Report Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX(+) cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato(+) cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato(+) cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-022-03203-5. BioMed Central 2022-11-12 /pmc/articles/PMC9652995/ /pubmed/36371298 http://dx.doi.org/10.1186/s13287-022-03203-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Fujisawa, Yuki Ming, Lu Yamada, Daisuke Takao, Tomoka Takarada, Takeshi Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title | Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title_full | Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title_fullStr | Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title_full_unstemmed | Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title_short | Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system |
title_sort | establishment of a human pluripotent stem cell-derived mkx-td tomato reporter system |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9652995/ https://www.ncbi.nlm.nih.gov/pubmed/36371298 http://dx.doi.org/10.1186/s13287-022-03203-5 |
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