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Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation

Dynamin is one of the major proteins involved in endocytosis. First identified 50 years ago in a genetic screen in Drosophila melanogaster, it has become a central player in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis, as well as other important c...

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Autor principal: Perrais, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653092/
https://www.ncbi.nlm.nih.gov/pubmed/36156116
http://dx.doi.org/10.1042/BSR20211227
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author Perrais, David
author_facet Perrais, David
author_sort Perrais, David
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description Dynamin is one of the major proteins involved in endocytosis. First identified 50 years ago in a genetic screen in Drosophila melanogaster, it has become a central player in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis, as well as other important cellular processes such as actin remodelling. Decades of work using biochemical and structural studies, cell-free assays, live cell imaging, acute inhibition and genetic studies have led to important insights on its mode of action. Dynamin is a remarkable mechano-GTPase, which can do a lot to membranes on its own but which is, in cells, at the centre of a vast protein and lipid network and cannot work in isolation. This review summarizes the main features of dynamin structure and function and its central role in membrane remodelling events, and give an update on the latest results.
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spelling pubmed-96530922022-11-18 Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation Perrais, David Biosci Rep Cell Membranes, Excitation & Transport Dynamin is one of the major proteins involved in endocytosis. First identified 50 years ago in a genetic screen in Drosophila melanogaster, it has become a central player in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis, as well as other important cellular processes such as actin remodelling. Decades of work using biochemical and structural studies, cell-free assays, live cell imaging, acute inhibition and genetic studies have led to important insights on its mode of action. Dynamin is a remarkable mechano-GTPase, which can do a lot to membranes on its own but which is, in cells, at the centre of a vast protein and lipid network and cannot work in isolation. This review summarizes the main features of dynamin structure and function and its central role in membrane remodelling events, and give an update on the latest results. Portland Press Ltd. 2022-11-10 /pmc/articles/PMC9653092/ /pubmed/36156116 http://dx.doi.org/10.1042/BSR20211227 Text en © 2022 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Cell Membranes, Excitation & Transport
Perrais, David
Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title_full Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title_fullStr Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title_full_unstemmed Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title_short Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
title_sort cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation
topic Cell Membranes, Excitation & Transport
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653092/
https://www.ncbi.nlm.nih.gov/pubmed/36156116
http://dx.doi.org/10.1042/BSR20211227
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