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At the molecular resolution with MINFLUX?

MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the...

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Detalles Bibliográficos
Autor principal: Prakash, Kirti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653251/
https://www.ncbi.nlm.nih.gov/pubmed/35152756
http://dx.doi.org/10.1098/rsta.2020.0145
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author Prakash, Kirti
author_facet Prakash, Kirti
author_sort Prakash, Kirti
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description MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’.
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spelling pubmed-96532512022-11-14 At the molecular resolution with MINFLUX? Prakash, Kirti Philos Trans A Math Phys Eng Sci Articles MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’. The Royal Society 2022-04-04 2022-02-14 /pmc/articles/PMC9653251/ /pubmed/35152756 http://dx.doi.org/10.1098/rsta.2020.0145 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Articles
Prakash, Kirti
At the molecular resolution with MINFLUX?
title At the molecular resolution with MINFLUX?
title_full At the molecular resolution with MINFLUX?
title_fullStr At the molecular resolution with MINFLUX?
title_full_unstemmed At the molecular resolution with MINFLUX?
title_short At the molecular resolution with MINFLUX?
title_sort at the molecular resolution with minflux?
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653251/
https://www.ncbi.nlm.nih.gov/pubmed/35152756
http://dx.doi.org/10.1098/rsta.2020.0145
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