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At the molecular resolution with MINFLUX?
MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653251/ https://www.ncbi.nlm.nih.gov/pubmed/35152756 http://dx.doi.org/10.1098/rsta.2020.0145 |
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author | Prakash, Kirti |
author_facet | Prakash, Kirti |
author_sort | Prakash, Kirti |
collection | PubMed |
description | MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’. |
format | Online Article Text |
id | pubmed-9653251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-96532512022-11-14 At the molecular resolution with MINFLUX? Prakash, Kirti Philos Trans A Math Phys Eng Sci Articles MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’. The Royal Society 2022-04-04 2022-02-14 /pmc/articles/PMC9653251/ /pubmed/35152756 http://dx.doi.org/10.1098/rsta.2020.0145 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Articles Prakash, Kirti At the molecular resolution with MINFLUX? |
title | At the molecular resolution with MINFLUX? |
title_full | At the molecular resolution with MINFLUX? |
title_fullStr | At the molecular resolution with MINFLUX? |
title_full_unstemmed | At the molecular resolution with MINFLUX? |
title_short | At the molecular resolution with MINFLUX? |
title_sort | at the molecular resolution with minflux? |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653251/ https://www.ncbi.nlm.nih.gov/pubmed/35152756 http://dx.doi.org/10.1098/rsta.2020.0145 |
work_keys_str_mv | AT prakashkirti atthemolecularresolutionwithminflux |