N and N′-mediated recognition confers resistance to tomato brown rugose fruit virus

Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus that overcomes the Tm-2 (2) resistance gene used in commercial tomato plants to protect against tobamoviruses. In this article, we show that ToBRFV is recognised through its P50 replicase fragment by the resistance gene N in N. tabacum , which triggers a hypersensitive response (HR). We also demonstrate that the N’ gene provides protection against ToBRFV through recognition of the viral coat protein without triggering a typical HR in N. tabacum .