Cargando…

Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases

Retinal organoids are three-dimensional (3D) structures derived from human pluripotent stem cells (hPSCs) that mimic the retina’s spatial and temporal differentiation, making them useful as in vitro retinal development models. Retinal organoids can be assembled with brain organoids, the 3D self-asse...

Descripción completa

Detalles Bibliográficos
Autores principales: Arthur, Peggy, Muok, Laureana, Nathani, Aakash, Zeng, Eric Z., Sun, Li, Li, Yan, Singh, Mandip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653705/
https://www.ncbi.nlm.nih.gov/pubmed/36359825
http://dx.doi.org/10.3390/cells11213429
_version_ 1784828746812882944
author Arthur, Peggy
Muok, Laureana
Nathani, Aakash
Zeng, Eric Z.
Sun, Li
Li, Yan
Singh, Mandip
author_facet Arthur, Peggy
Muok, Laureana
Nathani, Aakash
Zeng, Eric Z.
Sun, Li
Li, Yan
Singh, Mandip
author_sort Arthur, Peggy
collection PubMed
description Retinal organoids are three-dimensional (3D) structures derived from human pluripotent stem cells (hPSCs) that mimic the retina’s spatial and temporal differentiation, making them useful as in vitro retinal development models. Retinal organoids can be assembled with brain organoids, the 3D self-assembled aggregates derived from hPSCs containing different cell types and cytoarchitectures that resemble the human embryonic brain. Recent studies have shown the development of optic cups in brain organoids. The cellular components of a developing optic vesicle-containing organoids include primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. The importance of retinal organoids in ocular diseases such as age-related macular degeneration, Stargardt disease, retinitis pigmentosa, and diabetic retinopathy are described in this review. This review highlights current developments in retinal organoid techniques, and their applications in ocular conditions such as disease modeling, gene therapy, drug screening and development. In addition, recent advancements in utilizing extracellular vesicles secreted by retinal organoids for ocular disease treatments are summarized.
format Online
Article
Text
id pubmed-9653705
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96537052022-11-15 Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases Arthur, Peggy Muok, Laureana Nathani, Aakash Zeng, Eric Z. Sun, Li Li, Yan Singh, Mandip Cells Review Retinal organoids are three-dimensional (3D) structures derived from human pluripotent stem cells (hPSCs) that mimic the retina’s spatial and temporal differentiation, making them useful as in vitro retinal development models. Retinal organoids can be assembled with brain organoids, the 3D self-assembled aggregates derived from hPSCs containing different cell types and cytoarchitectures that resemble the human embryonic brain. Recent studies have shown the development of optic cups in brain organoids. The cellular components of a developing optic vesicle-containing organoids include primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. The importance of retinal organoids in ocular diseases such as age-related macular degeneration, Stargardt disease, retinitis pigmentosa, and diabetic retinopathy are described in this review. This review highlights current developments in retinal organoid techniques, and their applications in ocular conditions such as disease modeling, gene therapy, drug screening and development. In addition, recent advancements in utilizing extracellular vesicles secreted by retinal organoids for ocular disease treatments are summarized. MDPI 2022-10-30 /pmc/articles/PMC9653705/ /pubmed/36359825 http://dx.doi.org/10.3390/cells11213429 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Arthur, Peggy
Muok, Laureana
Nathani, Aakash
Zeng, Eric Z.
Sun, Li
Li, Yan
Singh, Mandip
Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title_full Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title_fullStr Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title_full_unstemmed Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title_short Bioengineering Human Pluripotent Stem Cell-Derived Retinal Organoids and Optic Vesicle-Containing Brain Organoids for Ocular Diseases
title_sort bioengineering human pluripotent stem cell-derived retinal organoids and optic vesicle-containing brain organoids for ocular diseases
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653705/
https://www.ncbi.nlm.nih.gov/pubmed/36359825
http://dx.doi.org/10.3390/cells11213429
work_keys_str_mv AT arthurpeggy bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT muoklaureana bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT nathaniaakash bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT zengericz bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT sunli bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT liyan bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases
AT singhmandip bioengineeringhumanpluripotentstemcellderivedretinalorganoidsandopticvesiclecontainingbrainorganoidsforoculardiseases