Cargando…
Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC)
Neuroinflammation following lipopolysaccharide (LPS) administration induces locomotor deficits and anxiety-like behaviour. In this study, minocycline was compared to memantine, an NMDA receptor antagonist, for its effects on LPS-induced locomotor deficits and anxiety-like behaviour in rats. Adult ma...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653752/ https://www.ncbi.nlm.nih.gov/pubmed/36362262 http://dx.doi.org/10.3390/ijms232113474 |
_version_ | 1784828758518136832 |
---|---|
author | Abdo Qaid, Entesar Yaseen Abdullah, Zuraidah Zakaria, Rahimah Long, Idris |
author_facet | Abdo Qaid, Entesar Yaseen Abdullah, Zuraidah Zakaria, Rahimah Long, Idris |
author_sort | Abdo Qaid, Entesar Yaseen |
collection | PubMed |
description | Neuroinflammation following lipopolysaccharide (LPS) administration induces locomotor deficits and anxiety-like behaviour. In this study, minocycline was compared to memantine, an NMDA receptor antagonist, for its effects on LPS-induced locomotor deficits and anxiety-like behaviour in rats. Adult male Sprague Dawley rats were administered either two different doses of minocycline (25 or 50 mg/kg/day, i.p.) or 10 mg/kg/day of memantine (i.p.) for 14 days four days prior to an LPS (5 mg/kg, i.p.) injection. Locomotor activity and anxiety-like behaviour were assessed using the open-field test (OFT). The phosphorylated tau protein level was measured using ELISA, while the expression and density of brain-derived neurotrophic factor (BDNF) and cAMP response element-binding (CREB) protein in the medial prefrontal cortex (mPFC) were measured using immunohistochemistry and Western blot, respectively. Minocycline treatment reduced locomotor deficits and anxiety-like behaviour associated with reduced phosphorylated tau protein levels, but it upregulated BDNF/CREB protein expressions in the mPFC in a comparable manner to memantine, with a higher dose of minocycline having better benefits. Minocycline treatment attenuated LPS-induced locomotor deficits and anxiety-like behaviour in rats and decreased phosphorylated tau protein levels, but it increased the expressions of the BDNF/CREB proteins in the mPFC. |
format | Online Article Text |
id | pubmed-9653752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96537522022-11-15 Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) Abdo Qaid, Entesar Yaseen Abdullah, Zuraidah Zakaria, Rahimah Long, Idris Int J Mol Sci Article Neuroinflammation following lipopolysaccharide (LPS) administration induces locomotor deficits and anxiety-like behaviour. In this study, minocycline was compared to memantine, an NMDA receptor antagonist, for its effects on LPS-induced locomotor deficits and anxiety-like behaviour in rats. Adult male Sprague Dawley rats were administered either two different doses of minocycline (25 or 50 mg/kg/day, i.p.) or 10 mg/kg/day of memantine (i.p.) for 14 days four days prior to an LPS (5 mg/kg, i.p.) injection. Locomotor activity and anxiety-like behaviour were assessed using the open-field test (OFT). The phosphorylated tau protein level was measured using ELISA, while the expression and density of brain-derived neurotrophic factor (BDNF) and cAMP response element-binding (CREB) protein in the medial prefrontal cortex (mPFC) were measured using immunohistochemistry and Western blot, respectively. Minocycline treatment reduced locomotor deficits and anxiety-like behaviour associated with reduced phosphorylated tau protein levels, but it upregulated BDNF/CREB protein expressions in the mPFC in a comparable manner to memantine, with a higher dose of minocycline having better benefits. Minocycline treatment attenuated LPS-induced locomotor deficits and anxiety-like behaviour in rats and decreased phosphorylated tau protein levels, but it increased the expressions of the BDNF/CREB proteins in the mPFC. MDPI 2022-11-03 /pmc/articles/PMC9653752/ /pubmed/36362262 http://dx.doi.org/10.3390/ijms232113474 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abdo Qaid, Entesar Yaseen Abdullah, Zuraidah Zakaria, Rahimah Long, Idris Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title | Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title_full | Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title_fullStr | Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title_full_unstemmed | Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title_short | Minocycline Attenuates Lipopolysaccharide-Induced Locomotor Deficit and Anxiety-like Behavior and Related Expression of the BDNF/CREB Protein in the Rat Medial Prefrontal Cortex (mPFC) |
title_sort | minocycline attenuates lipopolysaccharide-induced locomotor deficit and anxiety-like behavior and related expression of the bdnf/creb protein in the rat medial prefrontal cortex (mpfc) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653752/ https://www.ncbi.nlm.nih.gov/pubmed/36362262 http://dx.doi.org/10.3390/ijms232113474 |
work_keys_str_mv | AT abdoqaidentesaryaseen minocyclineattenuateslipopolysaccharideinducedlocomotordeficitandanxietylikebehaviorandrelatedexpressionofthebdnfcrebproteinintheratmedialprefrontalcortexmpfc AT abdullahzuraidah minocyclineattenuateslipopolysaccharideinducedlocomotordeficitandanxietylikebehaviorandrelatedexpressionofthebdnfcrebproteinintheratmedialprefrontalcortexmpfc AT zakariarahimah minocyclineattenuateslipopolysaccharideinducedlocomotordeficitandanxietylikebehaviorandrelatedexpressionofthebdnfcrebproteinintheratmedialprefrontalcortexmpfc AT longidris minocyclineattenuateslipopolysaccharideinducedlocomotordeficitandanxietylikebehaviorandrelatedexpressionofthebdnfcrebproteinintheratmedialprefrontalcortexmpfc |