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Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy

Changes in the expression of various genes, including pregnancy-associated hormone receptors and extracellular matrix proteins, have been suggested to play a significant role in bovine placental development. This study aimed to examine the influence of sex steroids and PGF(2α) on decorin (DCN) expre...

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Autores principales: Jamioł, Monika, Sozoniuk, Magdalena, Wawrzykowski, Jacek, Kankofer, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653824/
https://www.ncbi.nlm.nih.gov/pubmed/36364246
http://dx.doi.org/10.3390/molecules27217420
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author Jamioł, Monika
Sozoniuk, Magdalena
Wawrzykowski, Jacek
Kankofer, Marta
author_facet Jamioł, Monika
Sozoniuk, Magdalena
Wawrzykowski, Jacek
Kankofer, Marta
author_sort Jamioł, Monika
collection PubMed
description Changes in the expression of various genes, including pregnancy-associated hormone receptors and extracellular matrix proteins, have been suggested to play a significant role in bovine placental development. This study aimed to examine the influence of sex steroids and PGF(2α) on decorin (DCN) expression in the epithelial cells of bovine caruncle in early–mid pregnancy in cows. The expression patterns of DCN, PTGFR(,) PGR and ESR1 were analyzed by RT-qPCR and Western blotting in primary caruncular epithelial cell cultures (PCECC) and placental tissue homogenates derived from the 2nd and 4th months of pregnancy. PCECC were found to express DCN, PTGFR(,) PGR and ESR1. The intensity of PGR staining was higher in cells derived from the 4th month of pregnancy (p < 0.05). The 17β-estradiol, progesterone and PGF(2α) have not been shown to affect DCN expression. PGF(2α) decreased PTGFR expression in cells derived from the 4th month of gestation (p < 0.05). In conclusion, the results of the present preliminary study showed that the expression of the PTGFR, ESR1, PGR and DCN in PCECC does not vary throughout early–mid pregnancy. Further studies should be carried out to observe the relationship between hormonal status and cellular adhesion to determine their importance for properly developing placentation and pregnancy in cows.
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spelling pubmed-96538242022-11-15 Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy Jamioł, Monika Sozoniuk, Magdalena Wawrzykowski, Jacek Kankofer, Marta Molecules Article Changes in the expression of various genes, including pregnancy-associated hormone receptors and extracellular matrix proteins, have been suggested to play a significant role in bovine placental development. This study aimed to examine the influence of sex steroids and PGF(2α) on decorin (DCN) expression in the epithelial cells of bovine caruncle in early–mid pregnancy in cows. The expression patterns of DCN, PTGFR(,) PGR and ESR1 were analyzed by RT-qPCR and Western blotting in primary caruncular epithelial cell cultures (PCECC) and placental tissue homogenates derived from the 2nd and 4th months of pregnancy. PCECC were found to express DCN, PTGFR(,) PGR and ESR1. The intensity of PGR staining was higher in cells derived from the 4th month of pregnancy (p < 0.05). The 17β-estradiol, progesterone and PGF(2α) have not been shown to affect DCN expression. PGF(2α) decreased PTGFR expression in cells derived from the 4th month of gestation (p < 0.05). In conclusion, the results of the present preliminary study showed that the expression of the PTGFR, ESR1, PGR and DCN in PCECC does not vary throughout early–mid pregnancy. Further studies should be carried out to observe the relationship between hormonal status and cellular adhesion to determine their importance for properly developing placentation and pregnancy in cows. MDPI 2022-11-01 /pmc/articles/PMC9653824/ /pubmed/36364246 http://dx.doi.org/10.3390/molecules27217420 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jamioł, Monika
Sozoniuk, Magdalena
Wawrzykowski, Jacek
Kankofer, Marta
Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title_full Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title_fullStr Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title_full_unstemmed Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title_short Effect of Sex Steroids and PGF(2α) on the Expression of Their Receptors and Decorin in Bovine Caruncular Epithelial Cells in Early–Mid Pregnancy
title_sort effect of sex steroids and pgf(2α) on the expression of their receptors and decorin in bovine caruncular epithelial cells in early–mid pregnancy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9653824/
https://www.ncbi.nlm.nih.gov/pubmed/36364246
http://dx.doi.org/10.3390/molecules27217420
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