Cargando…

In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles

Silver nanoparticles (AgNPs) are widely used commercially due to their antimicrobial effects. Little is known about the effect of AgNPs on neural transmission and pain response. The aim of this study was to assess the anti-nociceptive activity of AgNPs. AgNPs were prepared at 16 ug/mL, white albino...

Descripción completa

Detalles Bibliográficos
Autores principales: Morsi, Shereen, Pittala, Valeria, Alqudah, Mohammad, Haider, Mohamed, Greish, Khaled
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654273/
https://www.ncbi.nlm.nih.gov/pubmed/36364085
http://dx.doi.org/10.3390/molecules27217259
_version_ 1784828889432850432
author Morsi, Shereen
Pittala, Valeria
Alqudah, Mohammad
Haider, Mohamed
Greish, Khaled
author_facet Morsi, Shereen
Pittala, Valeria
Alqudah, Mohammad
Haider, Mohamed
Greish, Khaled
author_sort Morsi, Shereen
collection PubMed
description Silver nanoparticles (AgNPs) are widely used commercially due to their antimicrobial effects. Little is known about the effect of AgNPs on neural transmission and pain response. The aim of this study was to assess the anti-nociceptive activity of AgNPs. AgNPs were prepared at 16 ug/mL, white albino rats were injected with various doses of AgNPs, and challenged using a hot-plate test and paw withdrawal latency (PWL) was measured. The chronic constriction injury (CCI) model was utilized to evaluate the pedal withdrawal reflex and tail withdrawal reflex. An electrophysiological study was conducted utilizing colon longitudinal muscle strips. AgNPs increased the latency of PWL in a dose-dependent matter over the duration of 6 h. The paw withdrawal threshold in animals with CCI significantly increased after AgNPs administration. In isolated colon longitudinal muscle strips, AgNPs significantly reduced the colonic migrating motor complexes (MMCs) and contraction. This action was completely reversed after removing the AgNPs and adding acetylcholine to the preparation. In this study, AgNPs showed significant anti-nociception properties. To our knowledge, this is the first report to describe this pharmacological action of AgNPs.
format Online
Article
Text
id pubmed-9654273
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96542732022-11-15 In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles Morsi, Shereen Pittala, Valeria Alqudah, Mohammad Haider, Mohamed Greish, Khaled Molecules Article Silver nanoparticles (AgNPs) are widely used commercially due to their antimicrobial effects. Little is known about the effect of AgNPs on neural transmission and pain response. The aim of this study was to assess the anti-nociceptive activity of AgNPs. AgNPs were prepared at 16 ug/mL, white albino rats were injected with various doses of AgNPs, and challenged using a hot-plate test and paw withdrawal latency (PWL) was measured. The chronic constriction injury (CCI) model was utilized to evaluate the pedal withdrawal reflex and tail withdrawal reflex. An electrophysiological study was conducted utilizing colon longitudinal muscle strips. AgNPs increased the latency of PWL in a dose-dependent matter over the duration of 6 h. The paw withdrawal threshold in animals with CCI significantly increased after AgNPs administration. In isolated colon longitudinal muscle strips, AgNPs significantly reduced the colonic migrating motor complexes (MMCs) and contraction. This action was completely reversed after removing the AgNPs and adding acetylcholine to the preparation. In this study, AgNPs showed significant anti-nociception properties. To our knowledge, this is the first report to describe this pharmacological action of AgNPs. MDPI 2022-10-26 /pmc/articles/PMC9654273/ /pubmed/36364085 http://dx.doi.org/10.3390/molecules27217259 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morsi, Shereen
Pittala, Valeria
Alqudah, Mohammad
Haider, Mohamed
Greish, Khaled
In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title_full In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title_fullStr In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title_full_unstemmed In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title_short In Vivo Evaluation of Anti-Nociceptive Effects of Silver Nanoparticles
title_sort in vivo evaluation of anti-nociceptive effects of silver nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654273/
https://www.ncbi.nlm.nih.gov/pubmed/36364085
http://dx.doi.org/10.3390/molecules27217259
work_keys_str_mv AT morsishereen invivoevaluationofantinociceptiveeffectsofsilvernanoparticles
AT pittalavaleria invivoevaluationofantinociceptiveeffectsofsilvernanoparticles
AT alqudahmohammad invivoevaluationofantinociceptiveeffectsofsilvernanoparticles
AT haidermohamed invivoevaluationofantinociceptiveeffectsofsilvernanoparticles
AT greishkhaled invivoevaluationofantinociceptiveeffectsofsilvernanoparticles