Cargando…

Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis

Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation descr...

Descripción completa

Detalles Bibliográficos
Autores principales: Sousa, Vanessa, Spratt, Dave, Davrandi, Mehmet, Mardas, Nikos, Beltrán, Víctor, Donos, Nikolaos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654334/
https://www.ncbi.nlm.nih.gov/pubmed/36360970
http://dx.doi.org/10.3390/ijerph192114088
_version_ 1784828905055584256
author Sousa, Vanessa
Spratt, Dave
Davrandi, Mehmet
Mardas, Nikos
Beltrán, Víctor
Donos, Nikolaos
author_facet Sousa, Vanessa
Spratt, Dave
Davrandi, Mehmet
Mardas, Nikos
Beltrán, Víctor
Donos, Nikolaos
author_sort Sousa, Vanessa
collection PubMed
description Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation describes the development of a novel in vitro microcosm biofilm model. Biofilms were grown over 30 days over machined titanium discs in a constant depth film fermentor (CDFF), which was inoculated (I) with pooled human saliva. Following longitudinal biofilm sampling across peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (PI) conditions, the characterisation of the biofilms was performed. The biofilm analyses included imaging by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), selective and non-selective culture media of viable biofilms, and 16S rRNA gene amplification and sequencing. Bacterial qualitative shifts were observed by CLSM and SEM across conditions, which were defined by characteristic phenotypes. A total of 9 phyla, 83 genera, and 156 species were identified throughout the experiment. The phyla Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria showed the highest prevalence in PI conditions. This novel in vitro microcosm model provides a high-throughput alternative for growing microcosm biofilms resembling an in vitro progression from PH–PM–PI conditions.
format Online
Article
Text
id pubmed-9654334
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96543342022-11-15 Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis Sousa, Vanessa Spratt, Dave Davrandi, Mehmet Mardas, Nikos Beltrán, Víctor Donos, Nikolaos Int J Environ Res Public Health Article Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation describes the development of a novel in vitro microcosm biofilm model. Biofilms were grown over 30 days over machined titanium discs in a constant depth film fermentor (CDFF), which was inoculated (I) with pooled human saliva. Following longitudinal biofilm sampling across peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (PI) conditions, the characterisation of the biofilms was performed. The biofilm analyses included imaging by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), selective and non-selective culture media of viable biofilms, and 16S rRNA gene amplification and sequencing. Bacterial qualitative shifts were observed by CLSM and SEM across conditions, which were defined by characteristic phenotypes. A total of 9 phyla, 83 genera, and 156 species were identified throughout the experiment. The phyla Proteobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Actinobacteria showed the highest prevalence in PI conditions. This novel in vitro microcosm model provides a high-throughput alternative for growing microcosm biofilms resembling an in vitro progression from PH–PM–PI conditions. MDPI 2022-10-28 /pmc/articles/PMC9654334/ /pubmed/36360970 http://dx.doi.org/10.3390/ijerph192114088 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sousa, Vanessa
Spratt, Dave
Davrandi, Mehmet
Mardas, Nikos
Beltrán, Víctor
Donos, Nikolaos
Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title_full Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title_fullStr Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title_full_unstemmed Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title_short Oral Microcosm Biofilms Grown under Conditions Progressing from Peri-Implant Health, Peri-Implant Mucositis, and Peri-Implantitis
title_sort oral microcosm biofilms grown under conditions progressing from peri-implant health, peri-implant mucositis, and peri-implantitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654334/
https://www.ncbi.nlm.nih.gov/pubmed/36360970
http://dx.doi.org/10.3390/ijerph192114088
work_keys_str_mv AT sousavanessa oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis
AT sprattdave oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis
AT davrandimehmet oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis
AT mardasnikos oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis
AT beltranvictor oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis
AT donosnikolaos oralmicrocosmbiofilmsgrownunderconditionsprogressingfromperiimplanthealthperiimplantmucositisandperiimplantitis