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Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts

Proper processing of natural material is crucial to obtain an extract with high content of biologically active components. Dried, grinded ginger roots were extracted by ultrasonic method and supercritical extraction with CO(2). The aim of the study was to determine if a mixture of the two types of e...

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Autores principales: Žitek, Taja, Kučuk, Nika, Postružnik, Vesna, Leitgeb, Maja, Knez, Željko, Primožič, Mateja, Marevci, Maša Knez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654478/
https://www.ncbi.nlm.nih.gov/pubmed/36365323
http://dx.doi.org/10.3390/plants11212872
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author Žitek, Taja
Kučuk, Nika
Postružnik, Vesna
Leitgeb, Maja
Knez, Željko
Primožič, Mateja
Marevci, Maša Knez
author_facet Žitek, Taja
Kučuk, Nika
Postružnik, Vesna
Leitgeb, Maja
Knez, Željko
Primožič, Mateja
Marevci, Maša Knez
author_sort Žitek, Taja
collection PubMed
description Proper processing of natural material is crucial to obtain an extract with high content of biologically active components. Dried, grinded ginger roots were extracted by ultrasonic method and supercritical extraction with CO(2). The aim of the study was to determine if a mixture of the two types of extracts attained by different methods and solvents exhibits better bioavailability than each extract itself. Therefore, both extracts were analytically evaluated and then mixed in a ratio of 1:1. The supercritical extract (SCG extract) and the mixed extract (mixG extract) had high antioxidant activity (78% and 73%) and total phenols (827 mg/g ext. and 1455 mg/g ext.), which is also consistent with the levels of gingerol (303 mg/g ext. and 271 g/g ext.) and shogaol (111 mg/g ext. and 100 g/g ext.) in the extracts. In comparison to both pure extracts higher levels of total phenols were found in the extract mixG. This could be the reason for the significant inhibition of melanoma cells and antimicrobial potential (against Staphylococcus aureus, Escherichia coli, and Candida albicans). The combination of the extracts resulted in a significant increase in the inhibition of selected microbial and melanoma cells WM-266-4 compared to the control. Cell viability decreased below 60% when mixG extract was applied. Antimicrobial activity has been confirmed.
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spelling pubmed-96544782022-11-15 Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts Žitek, Taja Kučuk, Nika Postružnik, Vesna Leitgeb, Maja Knez, Željko Primožič, Mateja Marevci, Maša Knez Plants (Basel) Article Proper processing of natural material is crucial to obtain an extract with high content of biologically active components. Dried, grinded ginger roots were extracted by ultrasonic method and supercritical extraction with CO(2). The aim of the study was to determine if a mixture of the two types of extracts attained by different methods and solvents exhibits better bioavailability than each extract itself. Therefore, both extracts were analytically evaluated and then mixed in a ratio of 1:1. The supercritical extract (SCG extract) and the mixed extract (mixG extract) had high antioxidant activity (78% and 73%) and total phenols (827 mg/g ext. and 1455 mg/g ext.), which is also consistent with the levels of gingerol (303 mg/g ext. and 271 g/g ext.) and shogaol (111 mg/g ext. and 100 g/g ext.) in the extracts. In comparison to both pure extracts higher levels of total phenols were found in the extract mixG. This could be the reason for the significant inhibition of melanoma cells and antimicrobial potential (against Staphylococcus aureus, Escherichia coli, and Candida albicans). The combination of the extracts resulted in a significant increase in the inhibition of selected microbial and melanoma cells WM-266-4 compared to the control. Cell viability decreased below 60% when mixG extract was applied. Antimicrobial activity has been confirmed. MDPI 2022-10-27 /pmc/articles/PMC9654478/ /pubmed/36365323 http://dx.doi.org/10.3390/plants11212872 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Žitek, Taja
Kučuk, Nika
Postružnik, Vesna
Leitgeb, Maja
Knez, Željko
Primožič, Mateja
Marevci, Maša Knez
Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title_full Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title_fullStr Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title_full_unstemmed Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title_short Synergistic Effect of Supercritical and Ultrasound-Assisted Ginger (Zingiber officinale Roscoe) Extracts
title_sort synergistic effect of supercritical and ultrasound-assisted ginger (zingiber officinale roscoe) extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654478/
https://www.ncbi.nlm.nih.gov/pubmed/36365323
http://dx.doi.org/10.3390/plants11212872
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