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Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality

Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/...

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Autores principales: Artini, Paolo Giovanni, Scarfò, Giorgia, Marzi, Ilaria, Fusi, Jonathan, Obino, Maria Elena, Franzoni, Ferdinando, Zappelli, Elisa, Chelucci, Elisa, Martini, Claudia, Cela, Vito, Daniele, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654514/
https://www.ncbi.nlm.nih.gov/pubmed/36362229
http://dx.doi.org/10.3390/ijms232113442
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author Artini, Paolo Giovanni
Scarfò, Giorgia
Marzi, Ilaria
Fusi, Jonathan
Obino, Maria Elena
Franzoni, Ferdinando
Zappelli, Elisa
Chelucci, Elisa
Martini, Claudia
Cela, Vito
Daniele, Simona
author_facet Artini, Paolo Giovanni
Scarfò, Giorgia
Marzi, Ilaria
Fusi, Jonathan
Obino, Maria Elena
Franzoni, Ferdinando
Zappelli, Elisa
Chelucci, Elisa
Martini, Claudia
Cela, Vito
Daniele, Simona
author_sort Artini, Paolo Giovanni
collection PubMed
description Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential.
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spelling pubmed-96545142022-11-15 Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality Artini, Paolo Giovanni Scarfò, Giorgia Marzi, Ilaria Fusi, Jonathan Obino, Maria Elena Franzoni, Ferdinando Zappelli, Elisa Chelucci, Elisa Martini, Claudia Cela, Vito Daniele, Simona Int J Mol Sci Article Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential. MDPI 2022-11-03 /pmc/articles/PMC9654514/ /pubmed/36362229 http://dx.doi.org/10.3390/ijms232113442 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Artini, Paolo Giovanni
Scarfò, Giorgia
Marzi, Ilaria
Fusi, Jonathan
Obino, Maria Elena
Franzoni, Ferdinando
Zappelli, Elisa
Chelucci, Elisa
Martini, Claudia
Cela, Vito
Daniele, Simona
Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title_full Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title_fullStr Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title_full_unstemmed Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title_short Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
title_sort oxidative stress-related signaling pathways predict oocytes’ fertilization in vitro and embryo quality
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654514/
https://www.ncbi.nlm.nih.gov/pubmed/36362229
http://dx.doi.org/10.3390/ijms232113442
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