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Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654514/ https://www.ncbi.nlm.nih.gov/pubmed/36362229 http://dx.doi.org/10.3390/ijms232113442 |
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author | Artini, Paolo Giovanni Scarfò, Giorgia Marzi, Ilaria Fusi, Jonathan Obino, Maria Elena Franzoni, Ferdinando Zappelli, Elisa Chelucci, Elisa Martini, Claudia Cela, Vito Daniele, Simona |
author_facet | Artini, Paolo Giovanni Scarfò, Giorgia Marzi, Ilaria Fusi, Jonathan Obino, Maria Elena Franzoni, Ferdinando Zappelli, Elisa Chelucci, Elisa Martini, Claudia Cela, Vito Daniele, Simona |
author_sort | Artini, Paolo Giovanni |
collection | PubMed |
description | Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential. |
format | Online Article Text |
id | pubmed-9654514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96545142022-11-15 Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality Artini, Paolo Giovanni Scarfò, Giorgia Marzi, Ilaria Fusi, Jonathan Obino, Maria Elena Franzoni, Ferdinando Zappelli, Elisa Chelucci, Elisa Martini, Claudia Cela, Vito Daniele, Simona Int J Mol Sci Article Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential. MDPI 2022-11-03 /pmc/articles/PMC9654514/ /pubmed/36362229 http://dx.doi.org/10.3390/ijms232113442 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Artini, Paolo Giovanni Scarfò, Giorgia Marzi, Ilaria Fusi, Jonathan Obino, Maria Elena Franzoni, Ferdinando Zappelli, Elisa Chelucci, Elisa Martini, Claudia Cela, Vito Daniele, Simona Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title | Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title_full | Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title_fullStr | Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title_full_unstemmed | Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title_short | Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality |
title_sort | oxidative stress-related signaling pathways predict oocytes’ fertilization in vitro and embryo quality |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654514/ https://www.ncbi.nlm.nih.gov/pubmed/36362229 http://dx.doi.org/10.3390/ijms232113442 |
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