Adipose Fin as a Natural “Optical Window” for Implantation of Fluorescent Sensors into Salmonid Fish

SIMPLE SUMMARY: Novel optical sensors require implantation into the most transparent organs in order to ensure the most reliable and rapid monitoring of animal health. Widely farmed salmonid fish, such as rainbow trout, have highly translucent adipose fin, which we tested here and showed its high potential as the implantation site for the fluorescent sensors. The filamentous sensors were convenient to inject into the fin, and their optical signal was easily detectable using a simple hand-held device even without immobilization of the fish. Responsiveness of the sensors inside the adipose fin to bodily changes was shown under induced acidosis of fish fluids. The obtained results characterize adipose fin as the favorable site for implantation of optical sensors into salmonids for real-time tracking animal physiological status in basic research and aquaculture. ABSTRACT: Implantable optical sensors are emerging tools that have the potential to enable constant real-time monitoring of various internal physiological parameters. Such a possibility will open new horizons for health control not only in medicine, but also in animal husbandry, including aquaculture. In this study, we analyze different organs of commonly farmed rainbow trout (Oncorhynchus mykiss) as implantation sites for fluorescent sensors and propose the adipose fin, lacking an endoskeleton, as the optimal choice. The fin is highly translucent due to significantly thinner dermis, which makes the detectable fluorescence of an implanted sensor operating at the visible light range by more than an order of magnitude higher relative to the skin. Compared to the proximal parts of ray fins, the adipose fin provides easy implantation and visualization of the sensor. Finally, we tested fluorescent pH sensors inside the adipose fin and demonstrated the possibility of acquiring their signal with a simple hand-held device and without fish anesthesia. All these features will most likely make the adipose fin the main “window” into the internal physiological processes of salmonid fish with the help of implantable optical sensors.