Simultaneous Identification of Functional Antigen-Specific CD8(+) and CD4(+) Cells after In Vitro Expansion Using Elongated Peptides

Elongated peptides (EPs), containing possibly one or multiple epitope/s, are increasingly used for the screening of antigen-specific CD8(+) and CD4(+) cell responses. Here, we present an in vitro protocol that allows the amplification of antigen-specific cells and the subsequent functional analysis...

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Detalles Bibliográficos
Autores principales: Schuhmacher, Juliane, Kleemann, Leon, Richardson, Jennifer Rebecca, Rusch, Elisa, Rammensee, Hans-Georg, Gouttefangeas, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654851/
https://www.ncbi.nlm.nih.gov/pubmed/36359847
http://dx.doi.org/10.3390/cells11213451

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654851/
https://www.ncbi.nlm.nih.gov/pubmed/36359847
http://dx.doi.org/10.3390/cells11213451

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