Stress Assessment of Wild Boar (Sus scrofa) in Corral-Style Traps Using Serum Cortisol Levels

SIMPLE SUMMARY: Corral-style traps for wild boar are used to reduce the number of wild boars. However, many people criticise these traps because of animal welfare issues such as stress and panic. While previous studies focused on behaviour and injuries, this study focused on the so-called stress hormone cortisol. Cortisol levels from trapped animals were compared with levels from animals shot during other hunting methods. Inside traps animals were killed by headshot within 2 h and 17 min after trapping and blood samples were directly taken. Cortisol levels were higher in wild boar killed in traps than in driven hunts and single hunts. Wild boar caught in groups of five or more showed lower cortisol levels than single animals or animals in smaller groups. Therefore, the time animals spend inside the trap and the time of culling all animals should be as short as possible. To reduce stress, it is better to capture larger groups of animals instead of single animals. For the evaluation of harm and stress for wild boar in live traps, cortisol levels alone are not sufficient. Additional information about the behaviour and injuries must be integrated. ABSTRACT: Capture of wild boar in corral-style traps with subsequent culling is increasingly used for population management. The method is debated due to animal welfare concerns making welfare studies in traps necessary. While previous studies focused on behaviour and injuries, this study dealt with the physiological aspect. Cortisol levels in wild boar caught in corral-style traps (50–90 qm(2), n = 138) were compared with those killed during single (n = 37) and driven hunts (n = 90). Collected sera were purified by solid phase extraction (SPE) and analysed via radioimmunoassay. Cortisol levels in blood samples were stable under cooled (4–7 °C) conditions for a storage time of up to 87 h before centrifugation. Cortisol levels were significantly higher in wild boar killed in corral-style traps than during driven hunts and single hunts. Wild boar caught in groups of five or more showed lower cortisol levels than single animals or in smaller groups. Therefore, time span inside the trap and of culling should be reduced to a minimum, and capturing groups of animals should be preferred to reduce stress. For animal welfare assessment of wild boar live-trapping, additional data from behavioural analyses and pathological examinations must be integrated.