Pros and Cons of In Vitro Methods for Circular RNA Preparation

mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advant...

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Detalles Bibliográficos
Autores principales: Lee, Kyung Hyun, Kim, Seongcheol, Lee, Seong-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654983/
https://www.ncbi.nlm.nih.gov/pubmed/36362032
http://dx.doi.org/10.3390/ijms232113247
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author Lee, Kyung Hyun
Kim, Seongcheol
Lee, Seong-Wook
author_facet Lee, Kyung Hyun
Kim, Seongcheol
Lee, Seong-Wook
author_sort Lee, Kyung Hyun
collection PubMed
description mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation.
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spelling pubmed-96549832022-11-15 Pros and Cons of In Vitro Methods for Circular RNA Preparation Lee, Kyung Hyun Kim, Seongcheol Lee, Seong-Wook Int J Mol Sci Review mRNA is gaining success as a new therapeutic agent and vaccine. However, mRNA has limitations in stability. To overcome the shortcomings of mRNA, circular RNA is emerging as a new modality. In this review, several current methods of manufacturing circular RNA in vitro are introduced and their advantages and disadvantages are reviewed. Furthermore, this study discusses which fields and directions of research and development are needed for the increase in the efficacy and productivity of circular RNA as a therapeutic agent and vaccine formulation. MDPI 2022-10-31 /pmc/articles/PMC9654983/ /pubmed/36362032 http://dx.doi.org/10.3390/ijms232113247 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lee, Kyung Hyun
Kim, Seongcheol
Lee, Seong-Wook
Pros and Cons of In Vitro Methods for Circular RNA Preparation
title Pros and Cons of In Vitro Methods for Circular RNA Preparation
title_full Pros and Cons of In Vitro Methods for Circular RNA Preparation
title_fullStr Pros and Cons of In Vitro Methods for Circular RNA Preparation
title_full_unstemmed Pros and Cons of In Vitro Methods for Circular RNA Preparation
title_short Pros and Cons of In Vitro Methods for Circular RNA Preparation
title_sort pros and cons of in vitro methods for circular rna preparation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654983/
https://www.ncbi.nlm.nih.gov/pubmed/36362032
http://dx.doi.org/10.3390/ijms232113247
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