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Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong

Ischemic stroke is a difficult-to-treat brain disease that may be attributed to a limited therapeutic time window and lack of effective clinical drugs. Nasal–brain administration is characterized by low systemic toxicity and is a direct and non-invasive brain targeting route. Preliminary studies hav...

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Autores principales: Huang, Chunhui, Wang, Canjian, Zhang, Wenliu, Yang, Tao, Xia, Mingyan, Lei, Xiaomeng, Peng, Ying, Wu, Yuhuan, Feng, Jianfang, Li, Dongxun, Zhang, Guosong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655233/
https://www.ncbi.nlm.nih.gov/pubmed/36364473
http://dx.doi.org/10.3390/molecules27217644
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author Huang, Chunhui
Wang, Canjian
Zhang, Wenliu
Yang, Tao
Xia, Mingyan
Lei, Xiaomeng
Peng, Ying
Wu, Yuhuan
Feng, Jianfang
Li, Dongxun
Zhang, Guosong
author_facet Huang, Chunhui
Wang, Canjian
Zhang, Wenliu
Yang, Tao
Xia, Mingyan
Lei, Xiaomeng
Peng, Ying
Wu, Yuhuan
Feng, Jianfang
Li, Dongxun
Zhang, Guosong
author_sort Huang, Chunhui
collection PubMed
description Ischemic stroke is a difficult-to-treat brain disease that may be attributed to a limited therapeutic time window and lack of effective clinical drugs. Nasal–brain administration is characterized by low systemic toxicity and is a direct and non-invasive brain targeting route. Preliminary studies have shown that the volatile oil of Chaxiong (VOC) has an obvious anti-ischemic stroke effect. In this work, we designed a nanoemulsion thermosensitive in situ gel (VOC-NE-ISG) loaded with volatile oil of Chaxiong for ischemia via intranasal delivery to rat brain treatment of cerebral ischemic stroke. The developed VOC-NE-ISG formulation has a suitable particle size of 21.02 ± 0.25 nm and a zeta potential of −20.4 ± 1.47 mV, with good gelling ability and prolonged release of the five components of VOC. The results of in vivo pharmacokinetic studies and brain targeting studies showed that intranasal administration of VOC-NE-ISG could significantly improve the bioavailability and had excellent brain-targeting efficacy of nasal-to-brain delivery. In addition, the results of pharmacodynamics experiments showed that both VOC-NE and VOC-NE-ISG could reduce the neurological deficit score of model rats, reducing the size of cerebral infarction, with a significant effect on improving ischemic stroke. Overall, VOC-NE-ISG may be a promising intranasal nanomedicine for the effective treatment of ischemic stroke.
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spelling pubmed-96552332022-11-15 Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong Huang, Chunhui Wang, Canjian Zhang, Wenliu Yang, Tao Xia, Mingyan Lei, Xiaomeng Peng, Ying Wu, Yuhuan Feng, Jianfang Li, Dongxun Zhang, Guosong Molecules Article Ischemic stroke is a difficult-to-treat brain disease that may be attributed to a limited therapeutic time window and lack of effective clinical drugs. Nasal–brain administration is characterized by low systemic toxicity and is a direct and non-invasive brain targeting route. Preliminary studies have shown that the volatile oil of Chaxiong (VOC) has an obvious anti-ischemic stroke effect. In this work, we designed a nanoemulsion thermosensitive in situ gel (VOC-NE-ISG) loaded with volatile oil of Chaxiong for ischemia via intranasal delivery to rat brain treatment of cerebral ischemic stroke. The developed VOC-NE-ISG formulation has a suitable particle size of 21.02 ± 0.25 nm and a zeta potential of −20.4 ± 1.47 mV, with good gelling ability and prolonged release of the five components of VOC. The results of in vivo pharmacokinetic studies and brain targeting studies showed that intranasal administration of VOC-NE-ISG could significantly improve the bioavailability and had excellent brain-targeting efficacy of nasal-to-brain delivery. In addition, the results of pharmacodynamics experiments showed that both VOC-NE and VOC-NE-ISG could reduce the neurological deficit score of model rats, reducing the size of cerebral infarction, with a significant effect on improving ischemic stroke. Overall, VOC-NE-ISG may be a promising intranasal nanomedicine for the effective treatment of ischemic stroke. MDPI 2022-11-07 /pmc/articles/PMC9655233/ /pubmed/36364473 http://dx.doi.org/10.3390/molecules27217644 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Chunhui
Wang, Canjian
Zhang, Wenliu
Yang, Tao
Xia, Mingyan
Lei, Xiaomeng
Peng, Ying
Wu, Yuhuan
Feng, Jianfang
Li, Dongxun
Zhang, Guosong
Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title_full Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title_fullStr Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title_full_unstemmed Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title_short Preparation, In Vitro and In Vivo Evaluation of Nanoemulsion In Situ Gel for Transnasal Delivery of Traditional Chinese Medicine Volatile Oil from Ligusticum sinense Oliv.cv. Chaxiong
title_sort preparation, in vitro and in vivo evaluation of nanoemulsion in situ gel for transnasal delivery of traditional chinese medicine volatile oil from ligusticum sinense oliv.cv. chaxiong
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655233/
https://www.ncbi.nlm.nih.gov/pubmed/36364473
http://dx.doi.org/10.3390/molecules27217644
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