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Improving Stability and Specificity of CRISPR/Cas9 System by Selective Modification of Guide RNAs with 2′-fluoro and Locked Nucleic Acid Nucleotides

The genome editing approach using the components of the CRISPR/Cas system has found wide application in molecular biology, fundamental medicine and genetic engineering. A promising method is to increase the efficacy and specificity of CRISPR/Cas-based genome editing systems by modifying their compon...

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Detalles Bibliográficos
Autores principales:	Sakovina, Lubov, Vokhtantsev, Ivan, Vorobyeva, Mariya, Vorobyev, Pavel, Novopashina, Darya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655745/ https://www.ncbi.nlm.nih.gov/pubmed/36362256 http://dx.doi.org/10.3390/ijms232113460

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