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The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) r...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655748/ https://www.ncbi.nlm.nih.gov/pubmed/36362654 http://dx.doi.org/10.3390/jcm11216421 |
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author | Zu, Lingling He, Jinling Zhou, Ning Zeng, Jingtong Zhu, Yifang Tang, Quanying Jin, Xin Zhang, Lei Xu, Song |
author_facet | Zu, Lingling He, Jinling Zhou, Ning Zeng, Jingtong Zhu, Yifang Tang, Quanying Jin, Xin Zhang, Lei Xu, Song |
author_sort | Zu, Lingling |
collection | PubMed |
description | Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) remain unclear. Here, we found that the genetic alterations in ITGB2 was predominated by gene mutation and copy number deletion using cBioPortal analysis, and its expression was downregulated in the NSCLC tissues, as validated by the UALCAN, TCGA, and GEO databases and our tissue samples. Kaplan–Meier (KM) plotter analysis revealed that patients with a lower ITGB2 expression had a shorter overall survival (OS) time (p = 0.01). Moreover, 1089 differentially expressed genes (DEGs) in the NSCLC tissues were screened using the TCGA database. The GO and KEGG enrichment analysis showed that the DEGs were closely associated with immune processes and cell adhesion. The protein–protein interaction (PPI) network revealed that 10 of 15 EMT-related genes among the DEGs might lead to the metastasis of NSCLC. Concomitantly, the expression of ITGB2 was positively correlated with the infiltration of Treg cells and Myeloid-derived suppressor cells (MDSC). Biologically, the ectopic expression of ITGB2 significantly inhibited the proliferation and metastasis of NSCLC cells. Mechanistically, we demonstrated that ITGB2 suppressed the expression of N-cadherin, Vimentin, Slug, Snail, and Twist, while it promoted E-cadherin expression, according to gain-of-function studies. In conclusion, ITGB2 can inhibit the proliferation and migration of NSCLC cells, leading to a poor prognosis, via epithelial–mesenchymal transition (EMT) signaling. |
format | Online Article Text |
id | pubmed-9655748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96557482022-11-15 The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer Zu, Lingling He, Jinling Zhou, Ning Zeng, Jingtong Zhu, Yifang Tang, Quanying Jin, Xin Zhang, Lei Xu, Song J Clin Med Article Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) remain unclear. Here, we found that the genetic alterations in ITGB2 was predominated by gene mutation and copy number deletion using cBioPortal analysis, and its expression was downregulated in the NSCLC tissues, as validated by the UALCAN, TCGA, and GEO databases and our tissue samples. Kaplan–Meier (KM) plotter analysis revealed that patients with a lower ITGB2 expression had a shorter overall survival (OS) time (p = 0.01). Moreover, 1089 differentially expressed genes (DEGs) in the NSCLC tissues were screened using the TCGA database. The GO and KEGG enrichment analysis showed that the DEGs were closely associated with immune processes and cell adhesion. The protein–protein interaction (PPI) network revealed that 10 of 15 EMT-related genes among the DEGs might lead to the metastasis of NSCLC. Concomitantly, the expression of ITGB2 was positively correlated with the infiltration of Treg cells and Myeloid-derived suppressor cells (MDSC). Biologically, the ectopic expression of ITGB2 significantly inhibited the proliferation and metastasis of NSCLC cells. Mechanistically, we demonstrated that ITGB2 suppressed the expression of N-cadherin, Vimentin, Slug, Snail, and Twist, while it promoted E-cadherin expression, according to gain-of-function studies. In conclusion, ITGB2 can inhibit the proliferation and migration of NSCLC cells, leading to a poor prognosis, via epithelial–mesenchymal transition (EMT) signaling. MDPI 2022-10-29 /pmc/articles/PMC9655748/ /pubmed/36362654 http://dx.doi.org/10.3390/jcm11216421 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zu, Lingling He, Jinling Zhou, Ning Zeng, Jingtong Zhu, Yifang Tang, Quanying Jin, Xin Zhang, Lei Xu, Song The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title | The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title_full | The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title_fullStr | The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title_full_unstemmed | The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title_short | The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer |
title_sort | profile and clinical significance of itgb2 expression in non-small-cell lung cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655748/ https://www.ncbi.nlm.nih.gov/pubmed/36362654 http://dx.doi.org/10.3390/jcm11216421 |
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