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The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer

Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) r...

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Autores principales: Zu, Lingling, He, Jinling, Zhou, Ning, Zeng, Jingtong, Zhu, Yifang, Tang, Quanying, Jin, Xin, Zhang, Lei, Xu, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655748/
https://www.ncbi.nlm.nih.gov/pubmed/36362654
http://dx.doi.org/10.3390/jcm11216421
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author Zu, Lingling
He, Jinling
Zhou, Ning
Zeng, Jingtong
Zhu, Yifang
Tang, Quanying
Jin, Xin
Zhang, Lei
Xu, Song
author_facet Zu, Lingling
He, Jinling
Zhou, Ning
Zeng, Jingtong
Zhu, Yifang
Tang, Quanying
Jin, Xin
Zhang, Lei
Xu, Song
author_sort Zu, Lingling
collection PubMed
description Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) remain unclear. Here, we found that the genetic alterations in ITGB2 was predominated by gene mutation and copy number deletion using cBioPortal analysis, and its expression was downregulated in the NSCLC tissues, as validated by the UALCAN, TCGA, and GEO databases and our tissue samples. Kaplan–Meier (KM) plotter analysis revealed that patients with a lower ITGB2 expression had a shorter overall survival (OS) time (p = 0.01). Moreover, 1089 differentially expressed genes (DEGs) in the NSCLC tissues were screened using the TCGA database. The GO and KEGG enrichment analysis showed that the DEGs were closely associated with immune processes and cell adhesion. The protein–protein interaction (PPI) network revealed that 10 of 15 EMT-related genes among the DEGs might lead to the metastasis of NSCLC. Concomitantly, the expression of ITGB2 was positively correlated with the infiltration of Treg cells and Myeloid-derived suppressor cells (MDSC). Biologically, the ectopic expression of ITGB2 significantly inhibited the proliferation and metastasis of NSCLC cells. Mechanistically, we demonstrated that ITGB2 suppressed the expression of N-cadherin, Vimentin, Slug, Snail, and Twist, while it promoted E-cadherin expression, according to gain-of-function studies. In conclusion, ITGB2 can inhibit the proliferation and migration of NSCLC cells, leading to a poor prognosis, via epithelial–mesenchymal transition (EMT) signaling.
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spelling pubmed-96557482022-11-15 The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer Zu, Lingling He, Jinling Zhou, Ning Zeng, Jingtong Zhu, Yifang Tang, Quanying Jin, Xin Zhang, Lei Xu, Song J Clin Med Article Integrins are involved in extracellular and intracellular signaling and are often aberrantly expressed in tumors. Integrin beta 2 (ITGB2) has previously been demonstrated to be correlated with the host defense. However, the expression profile and role of ITGB2 in non-small-cell lung cancer (NSCLC) remain unclear. Here, we found that the genetic alterations in ITGB2 was predominated by gene mutation and copy number deletion using cBioPortal analysis, and its expression was downregulated in the NSCLC tissues, as validated by the UALCAN, TCGA, and GEO databases and our tissue samples. Kaplan–Meier (KM) plotter analysis revealed that patients with a lower ITGB2 expression had a shorter overall survival (OS) time (p = 0.01). Moreover, 1089 differentially expressed genes (DEGs) in the NSCLC tissues were screened using the TCGA database. The GO and KEGG enrichment analysis showed that the DEGs were closely associated with immune processes and cell adhesion. The protein–protein interaction (PPI) network revealed that 10 of 15 EMT-related genes among the DEGs might lead to the metastasis of NSCLC. Concomitantly, the expression of ITGB2 was positively correlated with the infiltration of Treg cells and Myeloid-derived suppressor cells (MDSC). Biologically, the ectopic expression of ITGB2 significantly inhibited the proliferation and metastasis of NSCLC cells. Mechanistically, we demonstrated that ITGB2 suppressed the expression of N-cadherin, Vimentin, Slug, Snail, and Twist, while it promoted E-cadherin expression, according to gain-of-function studies. In conclusion, ITGB2 can inhibit the proliferation and migration of NSCLC cells, leading to a poor prognosis, via epithelial–mesenchymal transition (EMT) signaling. MDPI 2022-10-29 /pmc/articles/PMC9655748/ /pubmed/36362654 http://dx.doi.org/10.3390/jcm11216421 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zu, Lingling
He, Jinling
Zhou, Ning
Zeng, Jingtong
Zhu, Yifang
Tang, Quanying
Jin, Xin
Zhang, Lei
Xu, Song
The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title_full The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title_fullStr The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title_full_unstemmed The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title_short The Profile and Clinical Significance of ITGB2 Expression in Non-Small-Cell Lung Cancer
title_sort profile and clinical significance of itgb2 expression in non-small-cell lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655748/
https://www.ncbi.nlm.nih.gov/pubmed/36362654
http://dx.doi.org/10.3390/jcm11216421
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