Cargando…
Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors
Organ-on-chip (OoC) technology is one of the most promising in vitro tools to replace the traditional animal experiment-based paradigms of risk assessment. However, the use of OoC in drug discovery and toxicity studies remain still limited by the low capacity for high-throughput production and the i...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655789/ https://www.ncbi.nlm.nih.gov/pubmed/36365472 http://dx.doi.org/10.3390/polym14214478 |
_version_ | 1784829272693669888 |
---|---|
author | Etxeberria, Leire Messelmani, Taha Badiola, Jon Haitz Llobera, Andreu Fernandez, Luis Vilas-Vilela, José Luis Leclerc, Eric Legallais, Cécile Jellali, Rachid Zaldua, Ane Miren |
author_facet | Etxeberria, Leire Messelmani, Taha Badiola, Jon Haitz Llobera, Andreu Fernandez, Luis Vilas-Vilela, José Luis Leclerc, Eric Legallais, Cécile Jellali, Rachid Zaldua, Ane Miren |
author_sort | Etxeberria, Leire |
collection | PubMed |
description | Organ-on-chip (OoC) technology is one of the most promising in vitro tools to replace the traditional animal experiment-based paradigms of risk assessment. However, the use of OoC in drug discovery and toxicity studies remain still limited by the low capacity for high-throughput production and the incompatibility with standard laboratory equipment. Moreover, polydimethylsiloxanes, the material of choice for OoC, has several drawbacks, particularly the high absorption of drugs and chemicals. In this work, we report the development of a microfluidic device, using a process adapted for mass production, to culture liver cell line in dynamic conditions. The device, made of cyclic olefin copolymers, was manufactured by injection moulding and integrates Luer lock connectors compatible with standard medical and laboratory instruments. Then, the COC device was used for culturing HepG2/C3a cells. The functionality and behaviour of cultures were assessed by albumin secretion, cell proliferation, viability and actin cytoskeleton development. The cells in COC device proliferated well and remained functional for 9 days of culture. Furthermore, HepG2/C3a cells in the COC biochips showed similar behaviour to cells in PDMS biochips. The present study provides a proof-of-concept for the use of COC biochip in liver cells culture and illustrate their potential to develop OoC. |
format | Online Article Text |
id | pubmed-9655789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96557892022-11-15 Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors Etxeberria, Leire Messelmani, Taha Badiola, Jon Haitz Llobera, Andreu Fernandez, Luis Vilas-Vilela, José Luis Leclerc, Eric Legallais, Cécile Jellali, Rachid Zaldua, Ane Miren Polymers (Basel) Article Organ-on-chip (OoC) technology is one of the most promising in vitro tools to replace the traditional animal experiment-based paradigms of risk assessment. However, the use of OoC in drug discovery and toxicity studies remain still limited by the low capacity for high-throughput production and the incompatibility with standard laboratory equipment. Moreover, polydimethylsiloxanes, the material of choice for OoC, has several drawbacks, particularly the high absorption of drugs and chemicals. In this work, we report the development of a microfluidic device, using a process adapted for mass production, to culture liver cell line in dynamic conditions. The device, made of cyclic olefin copolymers, was manufactured by injection moulding and integrates Luer lock connectors compatible with standard medical and laboratory instruments. Then, the COC device was used for culturing HepG2/C3a cells. The functionality and behaviour of cultures were assessed by albumin secretion, cell proliferation, viability and actin cytoskeleton development. The cells in COC device proliferated well and remained functional for 9 days of culture. Furthermore, HepG2/C3a cells in the COC biochips showed similar behaviour to cells in PDMS biochips. The present study provides a proof-of-concept for the use of COC biochip in liver cells culture and illustrate their potential to develop OoC. MDPI 2022-10-22 /pmc/articles/PMC9655789/ /pubmed/36365472 http://dx.doi.org/10.3390/polym14214478 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Etxeberria, Leire Messelmani, Taha Badiola, Jon Haitz Llobera, Andreu Fernandez, Luis Vilas-Vilela, José Luis Leclerc, Eric Legallais, Cécile Jellali, Rachid Zaldua, Ane Miren Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title_full | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title_fullStr | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title_full_unstemmed | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title_short | Validation of HepG2/C3A Cell Cultures in Cyclic Olefin Copolymer Based Microfluidic Bioreactors |
title_sort | validation of hepg2/c3a cell cultures in cyclic olefin copolymer based microfluidic bioreactors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655789/ https://www.ncbi.nlm.nih.gov/pubmed/36365472 http://dx.doi.org/10.3390/polym14214478 |
work_keys_str_mv | AT etxeberrialeire validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT messelmanitaha validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT badiolajonhaitz validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT lloberaandreu validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT fernandezluis validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT vilasvilelajoseluis validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT leclerceric validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT legallaiscecile validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT jellalirachid validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors AT zalduaanemiren validationofhepg2c3acellculturesincyclicolefincopolymerbasedmicrofluidicbioreactors |