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Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations

This manuscript aimed to optimise the encapsulation of Thymus capitatus essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95. The optimal nanoemulsion showed nanometer-sized droplets (380 nm...

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Autores principales: BenJemaa, Mariem, Rahali, Fatma Zohra, Falleh, Hanen, Beji-Serairi, Raja, Megdiche-Ksouri, Wided, Hamrouni, Ibtissem, Hammami, Majdi, Nehme, Ralph, Pereira, Renato B., Andrés, Sonia, Bouhallab, Said, Pereira, David M., Ceciliani, Fabrizio, Abdennebi-Najar, Latifa, Ksouri, Riadh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655826/
https://www.ncbi.nlm.nih.gov/pubmed/36364161
http://dx.doi.org/10.3390/molecules27217330
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author BenJemaa, Mariem
Rahali, Fatma Zohra
Falleh, Hanen
Beji-Serairi, Raja
Megdiche-Ksouri, Wided
Hamrouni, Ibtissem
Hammami, Majdi
Nehme, Ralph
Pereira, Renato B.
Andrés, Sonia
Bouhallab, Said
Pereira, David M.
Ceciliani, Fabrizio
Abdennebi-Najar, Latifa
Ksouri, Riadh
author_facet BenJemaa, Mariem
Rahali, Fatma Zohra
Falleh, Hanen
Beji-Serairi, Raja
Megdiche-Ksouri, Wided
Hamrouni, Ibtissem
Hammami, Majdi
Nehme, Ralph
Pereira, Renato B.
Andrés, Sonia
Bouhallab, Said
Pereira, David M.
Ceciliani, Fabrizio
Abdennebi-Najar, Latifa
Ksouri, Riadh
author_sort BenJemaa, Mariem
collection PubMed
description This manuscript aimed to optimise the encapsulation of Thymus capitatus essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95. The optimal nanoemulsion showed nanometer-sized droplets (380 nm), a polydispersity index less than 0.5, and a suitable Zeta potential (−10.3 mV). Stability results showed that nanoemulsions stored at 4 °C were stable with the lowest d(3,2), PolyDispersity Index (PDI), and pH (day 11). Significant ameliorations in the capacity to neutralise DPPH radical after the encapsulation of the antimicrobial efficacy of thyme essential oil were recorded. S. typhimurium growth inhibition generated by nanoencapsulated thyme essential oil was 17 times higher than by bulk essential oil. The sensory analysis highlighted that the encapsulation of thyme essential oil improved enriched milk’s sensory appreciation. Indeed, 20% of the total population attributed a score of 4 and 5 on the scale used for milk enriched with nanoemulsion. In comparison, only 11% attributed the same score to milk enriched with bulk essential oil. The novel nanometric delivery system presents significant interest for agroalimentary industries.
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spelling pubmed-96558262022-11-15 Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations BenJemaa, Mariem Rahali, Fatma Zohra Falleh, Hanen Beji-Serairi, Raja Megdiche-Ksouri, Wided Hamrouni, Ibtissem Hammami, Majdi Nehme, Ralph Pereira, Renato B. Andrés, Sonia Bouhallab, Said Pereira, David M. Ceciliani, Fabrizio Abdennebi-Najar, Latifa Ksouri, Riadh Molecules Article This manuscript aimed to optimise the encapsulation of Thymus capitatus essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95. The optimal nanoemulsion showed nanometer-sized droplets (380 nm), a polydispersity index less than 0.5, and a suitable Zeta potential (−10.3 mV). Stability results showed that nanoemulsions stored at 4 °C were stable with the lowest d(3,2), PolyDispersity Index (PDI), and pH (day 11). Significant ameliorations in the capacity to neutralise DPPH radical after the encapsulation of the antimicrobial efficacy of thyme essential oil were recorded. S. typhimurium growth inhibition generated by nanoencapsulated thyme essential oil was 17 times higher than by bulk essential oil. The sensory analysis highlighted that the encapsulation of thyme essential oil improved enriched milk’s sensory appreciation. Indeed, 20% of the total population attributed a score of 4 and 5 on the scale used for milk enriched with nanoemulsion. In comparison, only 11% attributed the same score to milk enriched with bulk essential oil. The novel nanometric delivery system presents significant interest for agroalimentary industries. MDPI 2022-10-28 /pmc/articles/PMC9655826/ /pubmed/36364161 http://dx.doi.org/10.3390/molecules27217330 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
BenJemaa, Mariem
Rahali, Fatma Zohra
Falleh, Hanen
Beji-Serairi, Raja
Megdiche-Ksouri, Wided
Hamrouni, Ibtissem
Hammami, Majdi
Nehme, Ralph
Pereira, Renato B.
Andrés, Sonia
Bouhallab, Said
Pereira, David M.
Ceciliani, Fabrizio
Abdennebi-Najar, Latifa
Ksouri, Riadh
Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title_full Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title_fullStr Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title_full_unstemmed Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title_short Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations
title_sort essential oil stabilisation by response surface methodology (rsm): nanoemulsion formulation, physicochemical, microbiological, and sensory investigations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655826/
https://www.ncbi.nlm.nih.gov/pubmed/36364161
http://dx.doi.org/10.3390/molecules27217330
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