Cargando…

Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol

Dyslipidemias are one of the risk factors for cardiovascular diseases, the leading cause of death and hospitalization worldwide. One way to control cholesterol levels is to control the exogenous cholesterol intake in the body. Natural polyphenolic compounds, namely theaflavins from plant extracts su...

Descripción completa

Detalles Bibliográficos
Autores principales: Da Ressurreição, Sandrine, Pedreiro, Sónia, Batista, Maria Teresa, Figueirinha, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655935/
https://www.ncbi.nlm.nih.gov/pubmed/36364172
http://dx.doi.org/10.3390/molecules27217338
_version_ 1784829308662972416
author Da Ressurreição, Sandrine
Pedreiro, Sónia
Batista, Maria Teresa
Figueirinha, Artur
author_facet Da Ressurreição, Sandrine
Pedreiro, Sónia
Batista, Maria Teresa
Figueirinha, Artur
author_sort Da Ressurreição, Sandrine
collection PubMed
description Dyslipidemias are one of the risk factors for cardiovascular diseases, the leading cause of death and hospitalization worldwide. One way to control cholesterol levels is to control the exogenous cholesterol intake in the body. Natural polyphenolic compounds, namely theaflavins from plant extracts such as black tea, showed the ability to inhibit the formation of the micellar structure, essential for the absorption of cholesterol in the intestine. There are several methodologies to determine this effect, many of which are expensive and time-consuming. Due to these facts, the main purposes of this work were to optimize an inexpensive colorimetric method to study, in vitro, the micellar solubility of cholesterol and applied it to plant extracts. In this work, Cymbopogon citratus leaf extracts, its phenolic fractions, and flavonoids were evaluated. The non-delipidified infusion (CcI) obtained a maximum percentage of micelle destruction of 59.22% for a concentration of 50 μg/mL and the delipidified infusion (CcdI) obtained a maximum percentage of micelle destruction of 58.01% for a concentration of 200 μg/mL. In the case of the fraction of phenolic acids (CcPAs), 23.85% of maximum micellar destruction was recorded for the concentration of 100 μg/mL, while for the fraction of flavonoids (CcF), the micellar destruction was 92.74% at 1 μg/mL, and for the tannin fraction (CcT) of 99.45% at 25 μg/mL. Luteolin presented a percentage of micelle destruction of 94.83% in the concentration of 1 ng/mL, followed by luteolin-7-O-glucoside with 93.71% and luteo-lin-6-C-glucoside with 91.26% at the concentrations of 25 ng/mL and 50 ng/mL, respectively. These results suggest the capability of polyphenols from Cymbopogon citratus to prevent the cholesterol absorption in the gut by micellar destruction, and its contribution for cholesterol-lowering activity.
format Online
Article
Text
id pubmed-9655935
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96559352022-11-15 Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol Da Ressurreição, Sandrine Pedreiro, Sónia Batista, Maria Teresa Figueirinha, Artur Molecules Article Dyslipidemias are one of the risk factors for cardiovascular diseases, the leading cause of death and hospitalization worldwide. One way to control cholesterol levels is to control the exogenous cholesterol intake in the body. Natural polyphenolic compounds, namely theaflavins from plant extracts such as black tea, showed the ability to inhibit the formation of the micellar structure, essential for the absorption of cholesterol in the intestine. There are several methodologies to determine this effect, many of which are expensive and time-consuming. Due to these facts, the main purposes of this work were to optimize an inexpensive colorimetric method to study, in vitro, the micellar solubility of cholesterol and applied it to plant extracts. In this work, Cymbopogon citratus leaf extracts, its phenolic fractions, and flavonoids were evaluated. The non-delipidified infusion (CcI) obtained a maximum percentage of micelle destruction of 59.22% for a concentration of 50 μg/mL and the delipidified infusion (CcdI) obtained a maximum percentage of micelle destruction of 58.01% for a concentration of 200 μg/mL. In the case of the fraction of phenolic acids (CcPAs), 23.85% of maximum micellar destruction was recorded for the concentration of 100 μg/mL, while for the fraction of flavonoids (CcF), the micellar destruction was 92.74% at 1 μg/mL, and for the tannin fraction (CcT) of 99.45% at 25 μg/mL. Luteolin presented a percentage of micelle destruction of 94.83% in the concentration of 1 ng/mL, followed by luteolin-7-O-glucoside with 93.71% and luteo-lin-6-C-glucoside with 91.26% at the concentrations of 25 ng/mL and 50 ng/mL, respectively. These results suggest the capability of polyphenols from Cymbopogon citratus to prevent the cholesterol absorption in the gut by micellar destruction, and its contribution for cholesterol-lowering activity. MDPI 2022-10-28 /pmc/articles/PMC9655935/ /pubmed/36364172 http://dx.doi.org/10.3390/molecules27217338 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Da Ressurreição, Sandrine
Pedreiro, Sónia
Batista, Maria Teresa
Figueirinha, Artur
Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title_full Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title_fullStr Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title_full_unstemmed Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title_short Effect of Phenolic Compounds from Cymbopogon citratus (DC) Stapf. Leaves on Micellar Solubility of Cholesterol
title_sort effect of phenolic compounds from cymbopogon citratus (dc) stapf. leaves on micellar solubility of cholesterol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9655935/
https://www.ncbi.nlm.nih.gov/pubmed/36364172
http://dx.doi.org/10.3390/molecules27217338
work_keys_str_mv AT daressurreicaosandrine effectofphenoliccompoundsfromcymbopogoncitratusdcstapfleavesonmicellarsolubilityofcholesterol
AT pedreirosonia effectofphenoliccompoundsfromcymbopogoncitratusdcstapfleavesonmicellarsolubilityofcholesterol
AT batistamariateresa effectofphenoliccompoundsfromcymbopogoncitratusdcstapfleavesonmicellarsolubilityofcholesterol
AT figueirinhaartur effectofphenoliccompoundsfromcymbopogoncitratusdcstapfleavesonmicellarsolubilityofcholesterol