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A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application
In this study, a potential producer of prebiotics, a novel endo-polygalacturonase pePGA from Penicillium rolfsii BM-6, was successfully expressed in Komagataella phaffii, characterized and applied to produce pectic oligosaccharides. The optimum temperature and pH of pePGA were 60 °C and 6.0. The pur...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656037/ https://www.ncbi.nlm.nih.gov/pubmed/36360082 http://dx.doi.org/10.3390/foods11213469 |
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author | Hao, Meng-Jie Wu, Dan Xu, Yan Tao, Xiu-Mei Li, Ning Yu, Xiao-Wei |
author_facet | Hao, Meng-Jie Wu, Dan Xu, Yan Tao, Xiu-Mei Li, Ning Yu, Xiao-Wei |
author_sort | Hao, Meng-Jie |
collection | PubMed |
description | In this study, a potential producer of prebiotics, a novel endo-polygalacturonase pePGA from Penicillium rolfsii BM-6, was successfully expressed in Komagataella phaffii, characterized and applied to produce pectic oligosaccharides. The optimum temperature and pH of pePGA were 60 °C and 6.0. The purified recombinant enzyme showed a good pH stability and was stable from pH 3.5 to 8.0. The K(m), V(max) and k(cat) values of pePGA were 0.1569 g/L, 12,273 μmol/min/mg and 7478.4 s(−1), respectively. More importantly, pePGA-POS, the pePGA hydrolysis products from commercial pectin, had good prebiotic and antibacterial activities in vitro. The pePGA-POS was able to significantly promote the growth of probiotics; meanwhile, the growth of Escherichia coli JM109, Staphylococcus aureus and Bacillus subtilis 168 was effectively inhibited by pePGA-POS. In addition, pePGA-POS also had the DPPH radical scavenging capacity. These properties of pePGA-POS make pePGA attractive for the production of prebiotics. |
format | Online Article Text |
id | pubmed-9656037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96560372022-11-15 A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application Hao, Meng-Jie Wu, Dan Xu, Yan Tao, Xiu-Mei Li, Ning Yu, Xiao-Wei Foods Article In this study, a potential producer of prebiotics, a novel endo-polygalacturonase pePGA from Penicillium rolfsii BM-6, was successfully expressed in Komagataella phaffii, characterized and applied to produce pectic oligosaccharides. The optimum temperature and pH of pePGA were 60 °C and 6.0. The purified recombinant enzyme showed a good pH stability and was stable from pH 3.5 to 8.0. The K(m), V(max) and k(cat) values of pePGA were 0.1569 g/L, 12,273 μmol/min/mg and 7478.4 s(−1), respectively. More importantly, pePGA-POS, the pePGA hydrolysis products from commercial pectin, had good prebiotic and antibacterial activities in vitro. The pePGA-POS was able to significantly promote the growth of probiotics; meanwhile, the growth of Escherichia coli JM109, Staphylococcus aureus and Bacillus subtilis 168 was effectively inhibited by pePGA-POS. In addition, pePGA-POS also had the DPPH radical scavenging capacity. These properties of pePGA-POS make pePGA attractive for the production of prebiotics. MDPI 2022-11-01 /pmc/articles/PMC9656037/ /pubmed/36360082 http://dx.doi.org/10.3390/foods11213469 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hao, Meng-Jie Wu, Dan Xu, Yan Tao, Xiu-Mei Li, Ning Yu, Xiao-Wei A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title | A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title_full | A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title_fullStr | A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title_full_unstemmed | A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title_short | A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application |
title_sort | novel endo-polygalacturonase from penicillium rolfsii with prebiotics production potential: cloning, characterization and application |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656037/ https://www.ncbi.nlm.nih.gov/pubmed/36360082 http://dx.doi.org/10.3390/foods11213469 |
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