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Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis

We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation. In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus...

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Autores principales: Scanu, Anna, Luisetto, Roberto, Oliviero, Francesca, Galuppini, Francesca, Lazzarin, Vanni, Pennelli, Gianmaria, Masiero, Stefano, Punzi, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656099/
https://www.ncbi.nlm.nih.gov/pubmed/36361854
http://dx.doi.org/10.3390/ijms232113066
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author Scanu, Anna
Luisetto, Roberto
Oliviero, Francesca
Galuppini, Francesca
Lazzarin, Vanni
Pennelli, Gianmaria
Masiero, Stefano
Punzi, Leonardo
author_facet Scanu, Anna
Luisetto, Roberto
Oliviero, Francesca
Galuppini, Francesca
Lazzarin, Vanni
Pennelli, Gianmaria
Masiero, Stefano
Punzi, Leonardo
author_sort Scanu, Anna
collection PubMed
description We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation. In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus HA, twice a week for 2 months, and then euthanized to collect paw and blood. Arthritis was assessed in ankle joints by clinical and histological evaluation. Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA. After sacrifice, total and differential leukocyte counts were determined. Cytokine levels were measured in serum and peritoneal fluids. In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47–93%). In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35–74%). In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone. Results show that BPI attenuates CIA and inflammation in mice, and this effect is enhanced by HA co-administration. Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
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spelling pubmed-96560992022-11-15 Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis Scanu, Anna Luisetto, Roberto Oliviero, Francesca Galuppini, Francesca Lazzarin, Vanni Pennelli, Gianmaria Masiero, Stefano Punzi, Leonardo Int J Mol Sci Article We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation. In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus HA, twice a week for 2 months, and then euthanized to collect paw and blood. Arthritis was assessed in ankle joints by clinical and histological evaluation. Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA. After sacrifice, total and differential leukocyte counts were determined. Cytokine levels were measured in serum and peritoneal fluids. In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47–93%). In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35–74%). In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone. Results show that BPI attenuates CIA and inflammation in mice, and this effect is enhanced by HA co-administration. Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis. MDPI 2022-10-28 /pmc/articles/PMC9656099/ /pubmed/36361854 http://dx.doi.org/10.3390/ijms232113066 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scanu, Anna
Luisetto, Roberto
Oliviero, Francesca
Galuppini, Francesca
Lazzarin, Vanni
Pennelli, Gianmaria
Masiero, Stefano
Punzi, Leonardo
Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title_full Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title_fullStr Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title_full_unstemmed Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title_short Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
title_sort bactericidal/permeability-increasing protein downregulates the inflammatory response in in vivo models of arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656099/
https://www.ncbi.nlm.nih.gov/pubmed/36361854
http://dx.doi.org/10.3390/ijms232113066
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