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Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats

Diabetes mellitus (DM) is a chronic progressive metabolic disorder associated with several gastrointestinal complications, affecting up to 75% of patients. Knowing that Angiotensin II (AngII) also regulates intestinal contraction, we decided to evaluate changes in ileum and colon histomorphometry an...

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Autores principales: Esteves-Monteiro, Marisa, Menezes-Pinto, Daniela, Ferreira-Duarte, Mariana, Dias-Pereira, Patrícia, Morato, Manuela, Duarte-Araújo, Margarida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656372/
https://www.ncbi.nlm.nih.gov/pubmed/36362021
http://dx.doi.org/10.3390/ijms232113233
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author Esteves-Monteiro, Marisa
Menezes-Pinto, Daniela
Ferreira-Duarte, Mariana
Dias-Pereira, Patrícia
Morato, Manuela
Duarte-Araújo, Margarida
author_facet Esteves-Monteiro, Marisa
Menezes-Pinto, Daniela
Ferreira-Duarte, Mariana
Dias-Pereira, Patrícia
Morato, Manuela
Duarte-Araújo, Margarida
author_sort Esteves-Monteiro, Marisa
collection PubMed
description Diabetes mellitus (DM) is a chronic progressive metabolic disorder associated with several gastrointestinal complications, affecting up to 75% of patients. Knowing that Angiotensin II (AngII) also regulates intestinal contraction, we decided to evaluate changes in ileum and colon histomorphometry and AngII reactivity in a rat model of DM. Streptozotocin (STZ, 55 mg/kg) was administered to induce DM to 24 adult male Wistar rats. Diabetic rats displayed all the characteristic signs of type 1 DM (T1DM) and fecal excretion increased about 4-fold over 14 days, while the excretion of controls remained unaltered. Compared to controls, diabetic ileum and colon presented an increase in both macroscopic (length, perimeter and weight) and microscopic (muscular wall thickness) parameters. Functionally, AngII-induced smooth muscle contraction was lower in diabetic rats, except in the distal colon. These differences in the contractile response to AngII may result from an imbalance between AngII type 1 (antagonized by candesartan, 10 nM) and type 2 receptors activation (antagonized by PD123319, 100 nM). Taken together, these results indicate that an early and refined STZ-induced T1DM rat model already shows structural remodelling of the gut wall and decreased contractile response to AngII, findings that may help to explain diabetic dysmotility.
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spelling pubmed-96563722022-11-15 Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats Esteves-Monteiro, Marisa Menezes-Pinto, Daniela Ferreira-Duarte, Mariana Dias-Pereira, Patrícia Morato, Manuela Duarte-Araújo, Margarida Int J Mol Sci Article Diabetes mellitus (DM) is a chronic progressive metabolic disorder associated with several gastrointestinal complications, affecting up to 75% of patients. Knowing that Angiotensin II (AngII) also regulates intestinal contraction, we decided to evaluate changes in ileum and colon histomorphometry and AngII reactivity in a rat model of DM. Streptozotocin (STZ, 55 mg/kg) was administered to induce DM to 24 adult male Wistar rats. Diabetic rats displayed all the characteristic signs of type 1 DM (T1DM) and fecal excretion increased about 4-fold over 14 days, while the excretion of controls remained unaltered. Compared to controls, diabetic ileum and colon presented an increase in both macroscopic (length, perimeter and weight) and microscopic (muscular wall thickness) parameters. Functionally, AngII-induced smooth muscle contraction was lower in diabetic rats, except in the distal colon. These differences in the contractile response to AngII may result from an imbalance between AngII type 1 (antagonized by candesartan, 10 nM) and type 2 receptors activation (antagonized by PD123319, 100 nM). Taken together, these results indicate that an early and refined STZ-induced T1DM rat model already shows structural remodelling of the gut wall and decreased contractile response to AngII, findings that may help to explain diabetic dysmotility. MDPI 2022-10-31 /pmc/articles/PMC9656372/ /pubmed/36362021 http://dx.doi.org/10.3390/ijms232113233 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Esteves-Monteiro, Marisa
Menezes-Pinto, Daniela
Ferreira-Duarte, Mariana
Dias-Pereira, Patrícia
Morato, Manuela
Duarte-Araújo, Margarida
Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title_full Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title_fullStr Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title_full_unstemmed Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title_short Histomorphometry Changes and Decreased Reactivity to Angiotensin II in the Ileum and Colon of Streptozotocin-Induced Diabetic Rats
title_sort histomorphometry changes and decreased reactivity to angiotensin ii in the ileum and colon of streptozotocin-induced diabetic rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656372/
https://www.ncbi.nlm.nih.gov/pubmed/36362021
http://dx.doi.org/10.3390/ijms232113233
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