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Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta

Preeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several...

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Autores principales: Lombó, Marta, Giommi, Christian, Paolucci, Michela, Notarstefano, Valentina, Montik, Nina, Delli Carpini, Giovanni, Ciavattini, Andrea, Ragusa, Antonio, Maradonna, Francesca, Giorgini, Elisabetta, Carnevali, Oliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656520/
https://www.ncbi.nlm.nih.gov/pubmed/36361721
http://dx.doi.org/10.3390/ijms232112931
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author Lombó, Marta
Giommi, Christian
Paolucci, Michela
Notarstefano, Valentina
Montik, Nina
Delli Carpini, Giovanni
Ciavattini, Andrea
Ragusa, Antonio
Maradonna, Francesca
Giorgini, Elisabetta
Carnevali, Oliana
author_facet Lombó, Marta
Giommi, Christian
Paolucci, Michela
Notarstefano, Valentina
Montik, Nina
Delli Carpini, Giovanni
Ciavattini, Andrea
Ragusa, Antonio
Maradonna, Francesca
Giorgini, Elisabetta
Carnevali, Oliana
author_sort Lombó, Marta
collection PubMed
description Preeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several molecular alterations in the placental compartments with those occurring in the chorionic villi being of the utmost importance. Given the essential role of the endocannabinoid system during preimplantation and trophoblast migration, we have combined the histological and hyperspectral imaging analyses to shed light on the involvement of two cannabinoid receptors in the macromolecular alterations related to preeclampsia. The results obtained by immunohistochemistry showed a significant increase in the protein levels of cannabinoid receptor 1 (CB1) in the preeclamptic chorionic villi. However, no changes were reported regarding transient receptor potential vanilloid 1 (TRPV-1) levels either in the bulk placental samples or chorionic villi when comparing control and preeclamptic patients. Histological analysis and Fourier-transform infrared spectroscopy (FTIRI) showed an increase in collagen deposition together with higher levels of lipid peroxidation and phosphorylated compounds in the pathological villi. Since CB1 enhancement has been described as promoting fibrosis and oxidative stress in several tissues, we proposed that the higher receptor abundance in preeclampsia could be triggering similar molecular effects in preeclamptic term placentas.
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spelling pubmed-96565202022-11-15 Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta Lombó, Marta Giommi, Christian Paolucci, Michela Notarstefano, Valentina Montik, Nina Delli Carpini, Giovanni Ciavattini, Andrea Ragusa, Antonio Maradonna, Francesca Giorgini, Elisabetta Carnevali, Oliana Int J Mol Sci Article Preeclampsia is a human pregnancy-specific disease characterized by abnormal placentation that usually presents with maternal hypertension and proteinuria. The main hallmark of preeclampsia, impaired trophoblast migration, and the subsequent disruption of uterine arteries remodeling lead to several molecular alterations in the placental compartments with those occurring in the chorionic villi being of the utmost importance. Given the essential role of the endocannabinoid system during preimplantation and trophoblast migration, we have combined the histological and hyperspectral imaging analyses to shed light on the involvement of two cannabinoid receptors in the macromolecular alterations related to preeclampsia. The results obtained by immunohistochemistry showed a significant increase in the protein levels of cannabinoid receptor 1 (CB1) in the preeclamptic chorionic villi. However, no changes were reported regarding transient receptor potential vanilloid 1 (TRPV-1) levels either in the bulk placental samples or chorionic villi when comparing control and preeclamptic patients. Histological analysis and Fourier-transform infrared spectroscopy (FTIRI) showed an increase in collagen deposition together with higher levels of lipid peroxidation and phosphorylated compounds in the pathological villi. Since CB1 enhancement has been described as promoting fibrosis and oxidative stress in several tissues, we proposed that the higher receptor abundance in preeclampsia could be triggering similar molecular effects in preeclamptic term placentas. MDPI 2022-10-26 /pmc/articles/PMC9656520/ /pubmed/36361721 http://dx.doi.org/10.3390/ijms232112931 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lombó, Marta
Giommi, Christian
Paolucci, Michela
Notarstefano, Valentina
Montik, Nina
Delli Carpini, Giovanni
Ciavattini, Andrea
Ragusa, Antonio
Maradonna, Francesca
Giorgini, Elisabetta
Carnevali, Oliana
Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title_full Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title_fullStr Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title_full_unstemmed Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title_short Preeclampsia Correlates with an Increase in Cannabinoid Receptor 1 Levels Leading to Macromolecular Alterations in Chorionic Villi of Term Placenta
title_sort preeclampsia correlates with an increase in cannabinoid receptor 1 levels leading to macromolecular alterations in chorionic villi of term placenta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656520/
https://www.ncbi.nlm.nih.gov/pubmed/36361721
http://dx.doi.org/10.3390/ijms232112931
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