Phytochemical Composition and Antioxidant and Anti-Inflammatory Activities of Ligularia fischeri Turcz: A Comparison between Leaf and Root Extracts

Ligularia fischeri Turcz leaves are widely consumed and have multiple health benefits. We aimed to evaluate the differences in the phytochemical composition and biological properties of the root and leaf extracts from L. fischeri. The root extract exhibited higher antioxidant capacity and total flavonoid levels than the leaf extract. GC/MS analysis revealed the presence of various volatiles, diterpenoids, sesquiterpenes, and other non-polar compounds. Moreover, these extracts enhanced cellular antioxidant defense by reducing the level of reactive oxygen species and upregulating the expression of catalase and heme oxygenase-1 in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The root and leaf extracts also exerted anti-inflammatory effects by suppressing nitric oxide production and diminishing the levels of inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-1β in LPS-stimulated macrophages. Overall, these findings suggest that L. fischeri root extract contains diverse bioactive compounds for the development of nutraceuticals or functional foods with antioxidant and anti-inflammatory activity.