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From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction
The central nervous system (CNS) controls and regulates the functional activities of the organ systems and maintains the unity between the body and the external environment. The advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to repro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656609/ https://www.ncbi.nlm.nih.gov/pubmed/36361902 http://dx.doi.org/10.3390/ijms232113116 |
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author | Liu, Rongrong Meng, Xiaoting Yu, Xiyao Wang, Guoqiang Dong, Zhiyong Zhou, Zhengjie Qi, Mingran Yu, Xiao Ji, Tong Wang, Fang |
author_facet | Liu, Rongrong Meng, Xiaoting Yu, Xiyao Wang, Guoqiang Dong, Zhiyong Zhou, Zhengjie Qi, Mingran Yu, Xiao Ji, Tong Wang, Fang |
author_sort | Liu, Rongrong |
collection | PubMed |
description | The central nervous system (CNS) controls and regulates the functional activities of the organ systems and maintains the unity between the body and the external environment. The advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to reproduce complex neural circuits. Here, we classified the co-culture system as a two-dimensional (2D) co-culture system, a cell-based three-dimensional (3D) co-culture system, a tissue slice-based 3D co-culture system, an organoid-based 3D co-culture system, and a microfluidic platform-based 3D co-culture system. We provide an overview of these different co-culture models and their applications in the study of neural cell interaction. The application of co-culture systems in virus-infected CNS disease models is also discussed here. Finally, the direction of the co-culture system in future research is prospected. |
format | Online Article Text |
id | pubmed-9656609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96566092022-11-15 From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction Liu, Rongrong Meng, Xiaoting Yu, Xiyao Wang, Guoqiang Dong, Zhiyong Zhou, Zhengjie Qi, Mingran Yu, Xiao Ji, Tong Wang, Fang Int J Mol Sci Review The central nervous system (CNS) controls and regulates the functional activities of the organ systems and maintains the unity between the body and the external environment. The advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to reproduce complex neural circuits. Here, we classified the co-culture system as a two-dimensional (2D) co-culture system, a cell-based three-dimensional (3D) co-culture system, a tissue slice-based 3D co-culture system, an organoid-based 3D co-culture system, and a microfluidic platform-based 3D co-culture system. We provide an overview of these different co-culture models and their applications in the study of neural cell interaction. The application of co-culture systems in virus-infected CNS disease models is also discussed here. Finally, the direction of the co-culture system in future research is prospected. MDPI 2022-10-28 /pmc/articles/PMC9656609/ /pubmed/36361902 http://dx.doi.org/10.3390/ijms232113116 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Liu, Rongrong Meng, Xiaoting Yu, Xiyao Wang, Guoqiang Dong, Zhiyong Zhou, Zhengjie Qi, Mingran Yu, Xiao Ji, Tong Wang, Fang From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title | From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title_full | From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title_fullStr | From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title_full_unstemmed | From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title_short | From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction |
title_sort | from 2d to 3d co-culture systems: a review of co-culture models to study the neural cells interaction |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656609/ https://www.ncbi.nlm.nih.gov/pubmed/36361902 http://dx.doi.org/10.3390/ijms232113116 |
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