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Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis

In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effect...

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Detalles Bibliográficos
Autores principales: Guo, Yongxin, Chu, Guodong, Cai, Weijia, Li, Yaying, Lan, Xinxin, Li, Jing, Du, Linna, Yang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656666/
https://www.ncbi.nlm.nih.gov/pubmed/36361940
http://dx.doi.org/10.3390/ijms232113152
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author Guo, Yongxin
Chu, Guodong
Cai, Weijia
Li, Yaying
Lan, Xinxin
Li, Jing
Du, Linna
Yang, Jing
author_facet Guo, Yongxin
Chu, Guodong
Cai, Weijia
Li, Yaying
Lan, Xinxin
Li, Jing
Du, Linna
Yang, Jing
author_sort Guo, Yongxin
collection PubMed
description In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effects of oleosomes fused with human fibroblast growth factor 1 (OLAF) on wound healing. The data showed marked improvements in terms of the angiogenesis, vascular integrity, collagen and inflammation on the wound sites of rats with a full-thickness skin defect. Moreover, the positive role of OLAF in promoting angiogenesis and its possible pathways were clarified in vivo and in vitro. The results showed that the number, length and branches of the blood vessels of the chick embryo chorioallantoic membrane were markedly increased after OLAF treatment. Meanwhile, the in vitro results also revealed that 100 ng/mL OLAF exhibited a promoting effect on the proliferation, migration and tube formation of human umbilical vein endothelial cells. In addition, the potential of OLAF to improve wound angiogenesis was demonstrated to be associated with an up-regulated PI3K/Akt pathway by transcriptome sequencing analysis and the introduction of a PI3K/Akt pathway inhibitor (LY294002). These findings suggest that OLAF has many prospects in the development of drugs for wound healing.
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spelling pubmed-96566662022-11-15 Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis Guo, Yongxin Chu, Guodong Cai, Weijia Li, Yaying Lan, Xinxin Li, Jing Du, Linna Yang, Jing Int J Mol Sci Article In our previous study, human fibroblast growth factor 1 was successfully fused with oleosomes, energy-storing organelles of seeds, which are considered to be excellent “expression carriers” for substances with a convenient purification process. The present work aimed to explore the beneficial effects of oleosomes fused with human fibroblast growth factor 1 (OLAF) on wound healing. The data showed marked improvements in terms of the angiogenesis, vascular integrity, collagen and inflammation on the wound sites of rats with a full-thickness skin defect. Moreover, the positive role of OLAF in promoting angiogenesis and its possible pathways were clarified in vivo and in vitro. The results showed that the number, length and branches of the blood vessels of the chick embryo chorioallantoic membrane were markedly increased after OLAF treatment. Meanwhile, the in vitro results also revealed that 100 ng/mL OLAF exhibited a promoting effect on the proliferation, migration and tube formation of human umbilical vein endothelial cells. In addition, the potential of OLAF to improve wound angiogenesis was demonstrated to be associated with an up-regulated PI3K/Akt pathway by transcriptome sequencing analysis and the introduction of a PI3K/Akt pathway inhibitor (LY294002). These findings suggest that OLAF has many prospects in the development of drugs for wound healing. MDPI 2022-10-29 /pmc/articles/PMC9656666/ /pubmed/36361940 http://dx.doi.org/10.3390/ijms232113152 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guo, Yongxin
Chu, Guodong
Cai, Weijia
Li, Yaying
Lan, Xinxin
Li, Jing
Du, Linna
Yang, Jing
Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title_full Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title_fullStr Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title_full_unstemmed Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title_short Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis
title_sort beneficial effects of oleosomes fused with human fibroblast growth factor 1 on wound healing via the promotion of angiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656666/
https://www.ncbi.nlm.nih.gov/pubmed/36361940
http://dx.doi.org/10.3390/ijms232113152
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