Cargando…
Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis
RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of P. pe...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656875/ https://www.ncbi.nlm.nih.gov/pubmed/36364584 http://dx.doi.org/10.3390/nano12213809 |
_version_ | 1784829548438749184 |
---|---|
author | Wang, Zhenhui Li, Mingxia Kong, Ziyi Wang, Endong Zhang, Bo Lv, Jiale Xu, Xuenong |
author_facet | Wang, Zhenhui Li, Mingxia Kong, Ziyi Wang, Endong Zhang, Bo Lv, Jiale Xu, Xuenong |
author_sort | Wang, Zhenhui |
collection | PubMed |
description | RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of P. persimilis (PpATPb, PpATPd, PpRpL11, PpRpS2, and Pptra-2) were selected to verify whether SPc promotes the delivery of dsRNA into P. persimilis through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When PpATPb, PpATPd, PpRpL11, or PpRpS2 was interfered, both the fecundity and egg hatching rate decreased. In contrast, when Pptra-2 was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, P. persimilis expression of PpRpL11 reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites. |
format | Online Article Text |
id | pubmed-9656875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96568752022-11-15 Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis Wang, Zhenhui Li, Mingxia Kong, Ziyi Wang, Endong Zhang, Bo Lv, Jiale Xu, Xuenong Nanomaterials (Basel) Article RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of P. persimilis (PpATPb, PpATPd, PpRpL11, PpRpS2, and Pptra-2) were selected to verify whether SPc promotes the delivery of dsRNA into P. persimilis through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When PpATPb, PpATPd, PpRpL11, or PpRpS2 was interfered, both the fecundity and egg hatching rate decreased. In contrast, when Pptra-2 was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, P. persimilis expression of PpRpL11 reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites. MDPI 2022-10-28 /pmc/articles/PMC9656875/ /pubmed/36364584 http://dx.doi.org/10.3390/nano12213809 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Zhenhui Li, Mingxia Kong, Ziyi Wang, Endong Zhang, Bo Lv, Jiale Xu, Xuenong Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title_full | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title_fullStr | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title_full_unstemmed | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title_short | Star Polycation Mediated dsRNA Improves the Efficiency of RNA Interference in Phytoseiulus persimilis |
title_sort | star polycation mediated dsrna improves the efficiency of rna interference in phytoseiulus persimilis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656875/ https://www.ncbi.nlm.nih.gov/pubmed/36364584 http://dx.doi.org/10.3390/nano12213809 |
work_keys_str_mv | AT wangzhenhui starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT limingxia starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT kongziyi starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT wangendong starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT zhangbo starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT lvjiale starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis AT xuxuenong starpolycationmediateddsrnaimprovestheefficiencyofrnainterferenceinphytoseiuluspersimilis |