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An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients

Atopic dermatitis (AD), the most common inflammatory skin disorder, is a multifactorial disease characterized by a genetic predisposition, epidermal barrier disruption, a strong T helper (Th) type 2 immune reaction to environmental antigens and an altered cutaneous microbiome. Microbial dysbiosis ch...

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Autores principales: Cadau, Sébastien, Gault, Manon, Berthelemy, Nicolas, Hsu, Chiung-Yueh, Danoux, Louis, Pelletier, Nicolas, Goudounèche, Dominique, Pons, Carole, Leprince, Corinne, André-Frei, Valérie, Simon, Michel, Pain, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656979/
https://www.ncbi.nlm.nih.gov/pubmed/36361668
http://dx.doi.org/10.3390/ijms232112880
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author Cadau, Sébastien
Gault, Manon
Berthelemy, Nicolas
Hsu, Chiung-Yueh
Danoux, Louis
Pelletier, Nicolas
Goudounèche, Dominique
Pons, Carole
Leprince, Corinne
André-Frei, Valérie
Simon, Michel
Pain, Sabine
author_facet Cadau, Sébastien
Gault, Manon
Berthelemy, Nicolas
Hsu, Chiung-Yueh
Danoux, Louis
Pelletier, Nicolas
Goudounèche, Dominique
Pons, Carole
Leprince, Corinne
André-Frei, Valérie
Simon, Michel
Pain, Sabine
author_sort Cadau, Sébastien
collection PubMed
description Atopic dermatitis (AD), the most common inflammatory skin disorder, is a multifactorial disease characterized by a genetic predisposition, epidermal barrier disruption, a strong T helper (Th) type 2 immune reaction to environmental antigens and an altered cutaneous microbiome. Microbial dysbiosis characterized by the prevalence of Staphylococcus aureus (S. aureus) has been shown to exacerbate AD. In recent years, in vitro models of AD have been developed, but none of them reproduce all of the pathophysiological features. To better mimic AD, we developed reconstructed human epidermis (RHE) exposed to a Th2 pro-inflammatory cytokine cocktail and S. aureus. This model well reproduced some of the vicious loops involved in AD, with alterations at the physical, microbial and immune levels. Our results strongly suggest that S. aureus acquired a higher virulence potential when the epidermis was challenged with inflammatory cytokines, thus later contributing to the chronic inflammatory status. Furthermore, a topical application of a Castanea sativa extract was shown to prevent the apparition of the AD-like phenotype. It increased filaggrin, claudin-1 and loricrin expressions and controlled S. aureus by impairing its biofilm formation, enzymatic activities and inflammatory potential.
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spelling pubmed-96569792022-11-15 An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients Cadau, Sébastien Gault, Manon Berthelemy, Nicolas Hsu, Chiung-Yueh Danoux, Louis Pelletier, Nicolas Goudounèche, Dominique Pons, Carole Leprince, Corinne André-Frei, Valérie Simon, Michel Pain, Sabine Int J Mol Sci Article Atopic dermatitis (AD), the most common inflammatory skin disorder, is a multifactorial disease characterized by a genetic predisposition, epidermal barrier disruption, a strong T helper (Th) type 2 immune reaction to environmental antigens and an altered cutaneous microbiome. Microbial dysbiosis characterized by the prevalence of Staphylococcus aureus (S. aureus) has been shown to exacerbate AD. In recent years, in vitro models of AD have been developed, but none of them reproduce all of the pathophysiological features. To better mimic AD, we developed reconstructed human epidermis (RHE) exposed to a Th2 pro-inflammatory cytokine cocktail and S. aureus. This model well reproduced some of the vicious loops involved in AD, with alterations at the physical, microbial and immune levels. Our results strongly suggest that S. aureus acquired a higher virulence potential when the epidermis was challenged with inflammatory cytokines, thus later contributing to the chronic inflammatory status. Furthermore, a topical application of a Castanea sativa extract was shown to prevent the apparition of the AD-like phenotype. It increased filaggrin, claudin-1 and loricrin expressions and controlled S. aureus by impairing its biofilm formation, enzymatic activities and inflammatory potential. MDPI 2022-10-25 /pmc/articles/PMC9656979/ /pubmed/36361668 http://dx.doi.org/10.3390/ijms232112880 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cadau, Sébastien
Gault, Manon
Berthelemy, Nicolas
Hsu, Chiung-Yueh
Danoux, Louis
Pelletier, Nicolas
Goudounèche, Dominique
Pons, Carole
Leprince, Corinne
André-Frei, Valérie
Simon, Michel
Pain, Sabine
An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title_full An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title_fullStr An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title_full_unstemmed An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title_short An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients
title_sort inflamed and infected reconstructed human epidermis to study atopic dermatitis and skin care ingredients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656979/
https://www.ncbi.nlm.nih.gov/pubmed/36361668
http://dx.doi.org/10.3390/ijms232112880
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