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Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion
Cell-free hemoglobin (CFH), a pro-oxidant and cytotoxic compound that is released in hemolysis, has been associated with nephrotoxicity. Lung transplantation (LuTx) is a clinical condition with a high incidence of acute kidney injury (AKI). In this study, we investigated the plasma levels of CFH and...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657083/ https://www.ncbi.nlm.nih.gov/pubmed/36362059 http://dx.doi.org/10.3390/ijms232113272 |
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author | Greite, Robert Wang, Li Gohlke, Lukas Schott, Sebastian Kreimann, Kirill Doricic, Julian Leffler, Andreas Tudorache, Igor Salman, Jawad Natanov, Ruslan Ius, Fabio Fegbeutel, Christine Haverich, Axel Lichtinghagen, Ralf Chen, Rongjun Rong, Song Haller, Hermann Vijayan, Vijith Gram, Magnus Scheffner, Irina Gueler, Faikah Gwinner, Wilfried Immenschuh, Stephan |
author_facet | Greite, Robert Wang, Li Gohlke, Lukas Schott, Sebastian Kreimann, Kirill Doricic, Julian Leffler, Andreas Tudorache, Igor Salman, Jawad Natanov, Ruslan Ius, Fabio Fegbeutel, Christine Haverich, Axel Lichtinghagen, Ralf Chen, Rongjun Rong, Song Haller, Hermann Vijayan, Vijith Gram, Magnus Scheffner, Irina Gueler, Faikah Gwinner, Wilfried Immenschuh, Stephan |
author_sort | Greite, Robert |
collection | PubMed |
description | Cell-free hemoglobin (CFH), a pro-oxidant and cytotoxic compound that is released in hemolysis, has been associated with nephrotoxicity. Lung transplantation (LuTx) is a clinical condition with a high incidence of acute kidney injury (AKI). In this study, we investigated the plasma levels of CFH and haptoglobin, a CFH-binding serum protein, in prospectively enrolled LuTx patients (n = 20) with and without AKI. LuTx patients with postoperative AKI had higher CFH plasma levels at the end of surgery compared with no-AKI patients, and CFH correlated with serum creatinine at 48 h. Moreover, CFH levels inversely correlated with haptoglobin levels, which were significantly reduced at the end of surgery in LuTx patients with AKI. Because multiple other factors can contribute to AKI development in the complex clinical setting of LuTx, we next investigated the role of exogenous CFH administration in a mouse model of mild bilateral renal ischemia reperfusion injury (IRI). Exogenous administration of CFH after reperfusion caused overt AKI with creatinine increase, tubular injury, and enhanced markers of renal inflammation compared with vehicle-treated animals. In conclusion, CFH is a possible factor contributing to postoperative AKI after LuTx and promotes AKI in an experimental model of mild transient renal ischemia. Targeting CFH might be a therapeutic option to prevent AKI after LuTx. |
format | Online Article Text |
id | pubmed-9657083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96570832022-11-15 Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion Greite, Robert Wang, Li Gohlke, Lukas Schott, Sebastian Kreimann, Kirill Doricic, Julian Leffler, Andreas Tudorache, Igor Salman, Jawad Natanov, Ruslan Ius, Fabio Fegbeutel, Christine Haverich, Axel Lichtinghagen, Ralf Chen, Rongjun Rong, Song Haller, Hermann Vijayan, Vijith Gram, Magnus Scheffner, Irina Gueler, Faikah Gwinner, Wilfried Immenschuh, Stephan Int J Mol Sci Article Cell-free hemoglobin (CFH), a pro-oxidant and cytotoxic compound that is released in hemolysis, has been associated with nephrotoxicity. Lung transplantation (LuTx) is a clinical condition with a high incidence of acute kidney injury (AKI). In this study, we investigated the plasma levels of CFH and haptoglobin, a CFH-binding serum protein, in prospectively enrolled LuTx patients (n = 20) with and without AKI. LuTx patients with postoperative AKI had higher CFH plasma levels at the end of surgery compared with no-AKI patients, and CFH correlated with serum creatinine at 48 h. Moreover, CFH levels inversely correlated with haptoglobin levels, which were significantly reduced at the end of surgery in LuTx patients with AKI. Because multiple other factors can contribute to AKI development in the complex clinical setting of LuTx, we next investigated the role of exogenous CFH administration in a mouse model of mild bilateral renal ischemia reperfusion injury (IRI). Exogenous administration of CFH after reperfusion caused overt AKI with creatinine increase, tubular injury, and enhanced markers of renal inflammation compared with vehicle-treated animals. In conclusion, CFH is a possible factor contributing to postoperative AKI after LuTx and promotes AKI in an experimental model of mild transient renal ischemia. Targeting CFH might be a therapeutic option to prevent AKI after LuTx. MDPI 2022-10-31 /pmc/articles/PMC9657083/ /pubmed/36362059 http://dx.doi.org/10.3390/ijms232113272 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Greite, Robert Wang, Li Gohlke, Lukas Schott, Sebastian Kreimann, Kirill Doricic, Julian Leffler, Andreas Tudorache, Igor Salman, Jawad Natanov, Ruslan Ius, Fabio Fegbeutel, Christine Haverich, Axel Lichtinghagen, Ralf Chen, Rongjun Rong, Song Haller, Hermann Vijayan, Vijith Gram, Magnus Scheffner, Irina Gueler, Faikah Gwinner, Wilfried Immenschuh, Stephan Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title | Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title_full | Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title_fullStr | Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title_full_unstemmed | Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title_short | Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion |
title_sort | cell-free hemoglobin in acute kidney injury after lung transplantation and experimental renal ischemia/reperfusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657083/ https://www.ncbi.nlm.nih.gov/pubmed/36362059 http://dx.doi.org/10.3390/ijms232113272 |
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