Cargando…

Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy

We present a new photoswitchable spin label for light-induced pulsed electron paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of erythrosin B (EB), which is ideal for biological applications. With this label, we perform an in-depth study of the orientational effec...

Descripción completa

Detalles Bibliográficos
Autores principales: Bertran, Arnau, Morbiato, Laura, Aquilia, Sara, Gabbatore, Laura, De Zotti, Marta, Timmel, Christiane R., Di Valentin, Marilena, Bowen, Alice M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657417/
https://www.ncbi.nlm.nih.gov/pubmed/36364348
http://dx.doi.org/10.3390/molecules27217526
_version_ 1784829689103122432
author Bertran, Arnau
Morbiato, Laura
Aquilia, Sara
Gabbatore, Laura
De Zotti, Marta
Timmel, Christiane R.
Di Valentin, Marilena
Bowen, Alice M.
author_facet Bertran, Arnau
Morbiato, Laura
Aquilia, Sara
Gabbatore, Laura
De Zotti, Marta
Timmel, Christiane R.
Di Valentin, Marilena
Bowen, Alice M.
author_sort Bertran, Arnau
collection PubMed
description We present a new photoswitchable spin label for light-induced pulsed electron paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of erythrosin B (EB), which is ideal for biological applications. With this label, we perform an in-depth study of the orientational effects in dipolar traces acquired using the refocused laser-induced magnetic dipole technique to obtain information on the distance and relative orientation between the EB and nitroxide labels in a rigid model peptide, in good agreement with density functional theory predictions. Additionally, we show that these orientational effects can be averaged to enable an orientation-independent analysis to determine the distance distribution. Furthermore, we demonstrate the feasibility of these experiments above liquid nitrogen temperatures, removing the need for expensive liquid helium or cryogen-free cryostats. The variety of choices in photoswitchable spin labels and the affordability of the experiments are critical for LiPDS to become a widespread methodology in structural biology.
format Online
Article
Text
id pubmed-9657417
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96574172022-11-15 Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy Bertran, Arnau Morbiato, Laura Aquilia, Sara Gabbatore, Laura De Zotti, Marta Timmel, Christiane R. Di Valentin, Marilena Bowen, Alice M. Molecules Communication We present a new photoswitchable spin label for light-induced pulsed electron paramagnetic resonance dipolar spectroscopy (LiPDS), the photoexcited triplet state of erythrosin B (EB), which is ideal for biological applications. With this label, we perform an in-depth study of the orientational effects in dipolar traces acquired using the refocused laser-induced magnetic dipole technique to obtain information on the distance and relative orientation between the EB and nitroxide labels in a rigid model peptide, in good agreement with density functional theory predictions. Additionally, we show that these orientational effects can be averaged to enable an orientation-independent analysis to determine the distance distribution. Furthermore, we demonstrate the feasibility of these experiments above liquid nitrogen temperatures, removing the need for expensive liquid helium or cryogen-free cryostats. The variety of choices in photoswitchable spin labels and the affordability of the experiments are critical for LiPDS to become a widespread methodology in structural biology. MDPI 2022-11-03 /pmc/articles/PMC9657417/ /pubmed/36364348 http://dx.doi.org/10.3390/molecules27217526 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Bertran, Arnau
Morbiato, Laura
Aquilia, Sara
Gabbatore, Laura
De Zotti, Marta
Timmel, Christiane R.
Di Valentin, Marilena
Bowen, Alice M.
Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title_full Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title_fullStr Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title_full_unstemmed Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title_short Erythrosin B as a New Photoswitchable Spin Label for Light-Induced Pulsed EPR Dipolar Spectroscopy
title_sort erythrosin b as a new photoswitchable spin label for light-induced pulsed epr dipolar spectroscopy
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657417/
https://www.ncbi.nlm.nih.gov/pubmed/36364348
http://dx.doi.org/10.3390/molecules27217526
work_keys_str_mv AT bertranarnau erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT morbiatolaura erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT aquiliasara erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT gabbatorelaura erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT dezottimarta erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT timmelchristianer erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT divalentinmarilena erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy
AT bowenalicem erythrosinbasanewphotoswitchablespinlabelforlightinducedpulsedeprdipolarspectroscopy