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PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation

The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary...

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Autores principales: Brandt, Nicola, Willmer, Jan Philipp, Ayon-Olivas, Maurilyn S., Banicka, Veronika, Witt, Martin, Wree, Andreas, Groß, Isabel, Gläser, Anne, Hausmann, Jens, Bräuer, Anja U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657456/
https://www.ncbi.nlm.nih.gov/pubmed/36361802
http://dx.doi.org/10.3390/ijms232113007
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author Brandt, Nicola
Willmer, Jan Philipp
Ayon-Olivas, Maurilyn S.
Banicka, Veronika
Witt, Martin
Wree, Andreas
Groß, Isabel
Gläser, Anne
Hausmann, Jens
Bräuer, Anja U.
author_facet Brandt, Nicola
Willmer, Jan Philipp
Ayon-Olivas, Maurilyn S.
Banicka, Veronika
Witt, Martin
Wree, Andreas
Groß, Isabel
Gläser, Anne
Hausmann, Jens
Bräuer, Anja U.
author_sort Brandt, Nicola
collection PubMed
description The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary neurons; PRG5 contributes to spine induction in immature neurons and the regulation of spine density and morphology in mature neurons. Both exhibit intracellularly located C-termini of less than 50 amino acids. Varying C-termini suggested that these domains shape neuronal morphology differently. We generated mutant EGFP-fusion proteins in which the C-termini were either swapped between PRG3 and PRG5, deleted, or fused to another family member, plasticity-related gene 4 (PRG4), that was recently shown to be expressed in different brain regions. We subsequently analyzed the influence of overexpression in immature neurons. Our results point to a critical role of the PRG3 and PRG5 C-termini in shaping early neuronal morphology. However, the results suggest that the C-terminus alone might not be sufficient for promoting the morphological effects induced by PRG3 and PRG5.
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spelling pubmed-96574562022-11-15 PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation Brandt, Nicola Willmer, Jan Philipp Ayon-Olivas, Maurilyn S. Banicka, Veronika Witt, Martin Wree, Andreas Groß, Isabel Gläser, Anne Hausmann, Jens Bräuer, Anja U. Int J Mol Sci Article The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary neurons; PRG5 contributes to spine induction in immature neurons and the regulation of spine density and morphology in mature neurons. Both exhibit intracellularly located C-termini of less than 50 amino acids. Varying C-termini suggested that these domains shape neuronal morphology differently. We generated mutant EGFP-fusion proteins in which the C-termini were either swapped between PRG3 and PRG5, deleted, or fused to another family member, plasticity-related gene 4 (PRG4), that was recently shown to be expressed in different brain regions. We subsequently analyzed the influence of overexpression in immature neurons. Our results point to a critical role of the PRG3 and PRG5 C-termini in shaping early neuronal morphology. However, the results suggest that the C-terminus alone might not be sufficient for promoting the morphological effects induced by PRG3 and PRG5. MDPI 2022-10-27 /pmc/articles/PMC9657456/ /pubmed/36361802 http://dx.doi.org/10.3390/ijms232113007 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brandt, Nicola
Willmer, Jan Philipp
Ayon-Olivas, Maurilyn S.
Banicka, Veronika
Witt, Martin
Wree, Andreas
Groß, Isabel
Gläser, Anne
Hausmann, Jens
Bräuer, Anja U.
PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title_full PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title_fullStr PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title_full_unstemmed PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title_short PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
title_sort prg3 and prg5 c-termini: important players in early neuronal differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657456/
https://www.ncbi.nlm.nih.gov/pubmed/36361802
http://dx.doi.org/10.3390/ijms232113007
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