Cargando…
PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657456/ https://www.ncbi.nlm.nih.gov/pubmed/36361802 http://dx.doi.org/10.3390/ijms232113007 |
_version_ | 1784829698956591104 |
---|---|
author | Brandt, Nicola Willmer, Jan Philipp Ayon-Olivas, Maurilyn S. Banicka, Veronika Witt, Martin Wree, Andreas Groß, Isabel Gläser, Anne Hausmann, Jens Bräuer, Anja U. |
author_facet | Brandt, Nicola Willmer, Jan Philipp Ayon-Olivas, Maurilyn S. Banicka, Veronika Witt, Martin Wree, Andreas Groß, Isabel Gläser, Anne Hausmann, Jens Bräuer, Anja U. |
author_sort | Brandt, Nicola |
collection | PubMed |
description | The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary neurons; PRG5 contributes to spine induction in immature neurons and the regulation of spine density and morphology in mature neurons. Both exhibit intracellularly located C-termini of less than 50 amino acids. Varying C-termini suggested that these domains shape neuronal morphology differently. We generated mutant EGFP-fusion proteins in which the C-termini were either swapped between PRG3 and PRG5, deleted, or fused to another family member, plasticity-related gene 4 (PRG4), that was recently shown to be expressed in different brain regions. We subsequently analyzed the influence of overexpression in immature neurons. Our results point to a critical role of the PRG3 and PRG5 C-termini in shaping early neuronal morphology. However, the results suggest that the C-terminus alone might not be sufficient for promoting the morphological effects induced by PRG3 and PRG5. |
format | Online Article Text |
id | pubmed-9657456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96574562022-11-15 PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation Brandt, Nicola Willmer, Jan Philipp Ayon-Olivas, Maurilyn S. Banicka, Veronika Witt, Martin Wree, Andreas Groß, Isabel Gläser, Anne Hausmann, Jens Bräuer, Anja U. Int J Mol Sci Article The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary neurons; PRG5 contributes to spine induction in immature neurons and the regulation of spine density and morphology in mature neurons. Both exhibit intracellularly located C-termini of less than 50 amino acids. Varying C-termini suggested that these domains shape neuronal morphology differently. We generated mutant EGFP-fusion proteins in which the C-termini were either swapped between PRG3 and PRG5, deleted, or fused to another family member, plasticity-related gene 4 (PRG4), that was recently shown to be expressed in different brain regions. We subsequently analyzed the influence of overexpression in immature neurons. Our results point to a critical role of the PRG3 and PRG5 C-termini in shaping early neuronal morphology. However, the results suggest that the C-terminus alone might not be sufficient for promoting the morphological effects induced by PRG3 and PRG5. MDPI 2022-10-27 /pmc/articles/PMC9657456/ /pubmed/36361802 http://dx.doi.org/10.3390/ijms232113007 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brandt, Nicola Willmer, Jan Philipp Ayon-Olivas, Maurilyn S. Banicka, Veronika Witt, Martin Wree, Andreas Groß, Isabel Gläser, Anne Hausmann, Jens Bräuer, Anja U. PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title | PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title_full | PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title_fullStr | PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title_full_unstemmed | PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title_short | PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation |
title_sort | prg3 and prg5 c-termini: important players in early neuronal differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657456/ https://www.ncbi.nlm.nih.gov/pubmed/36361802 http://dx.doi.org/10.3390/ijms232113007 |
work_keys_str_mv | AT brandtnicola prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT willmerjanphilipp prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT ayonolivasmaurilyns prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT banickaveronika prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT wittmartin prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT wreeandreas prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT großisabel prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT glaseranne prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT hausmannjens prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation AT braueranjau prg3andprg5cterminiimportantplayersinearlyneuronaldifferentiation |