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The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches

Background: Cytotoxicity testing is a primary method to establish the safety of biomaterials, e.g., biocomposites. Biomaterials involve a wide range of medical materials, which are usually solid materials and are used in bone regeneration, cardiology, or dermatology. Current advancements in science...

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Autores principales: Chraniuk, Milena, Panasiuk, Mirosława, Hovhannisyan, Lilit, Żołędowska, Sabina, Nidzworski, Dawid, Ciołek, Lidia, Woźniak, Anna, Jaegermann, Zbigniew, Biernat, Monika, Gromadzka, Beata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657594/
https://www.ncbi.nlm.nih.gov/pubmed/36365516
http://dx.doi.org/10.3390/polym14214522
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author Chraniuk, Milena
Panasiuk, Mirosława
Hovhannisyan, Lilit
Żołędowska, Sabina
Nidzworski, Dawid
Ciołek, Lidia
Woźniak, Anna
Jaegermann, Zbigniew
Biernat, Monika
Gromadzka, Beata
author_facet Chraniuk, Milena
Panasiuk, Mirosława
Hovhannisyan, Lilit
Żołędowska, Sabina
Nidzworski, Dawid
Ciołek, Lidia
Woźniak, Anna
Jaegermann, Zbigniew
Biernat, Monika
Gromadzka, Beata
author_sort Chraniuk, Milena
collection PubMed
description Background: Cytotoxicity testing is a primary method to establish the safety of biomaterials, e.g., biocomposites. Biomaterials involve a wide range of medical materials, which are usually solid materials and are used in bone regeneration, cardiology, or dermatology. Current advancements in science and technology provide several standard cytotoxicity testing methods that are sufficiently sensitive to detect various levels of cellular toxicity, i.e., from low to high. The aim was to compare the direct and indirect methodology described in the ISO guidelines UNE-EN ISO 10993-5:2009 Part 5. Methods: Cell proliferation was measured using WST-1 assay, and cytotoxicity was measured using LDH test kit. Results: The results indicate that the molecular surface of biomaterials have impact on the cytotoxicity and proliferation profile. Based on these results, we confirm that the indirect method does not provide a clear picture of the cell condition after the exposure to the surface, and moreover, cannot provide complete results about the effects of the material. Conclusions: Comparison of both methods shows that it is pivotal to investigate biomaterials at the very early stages using both indirect and direct methods to access the influence of the released toxins and surface of the material on the cell condition.
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spelling pubmed-96575942022-11-15 The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches Chraniuk, Milena Panasiuk, Mirosława Hovhannisyan, Lilit Żołędowska, Sabina Nidzworski, Dawid Ciołek, Lidia Woźniak, Anna Jaegermann, Zbigniew Biernat, Monika Gromadzka, Beata Polymers (Basel) Article Background: Cytotoxicity testing is a primary method to establish the safety of biomaterials, e.g., biocomposites. Biomaterials involve a wide range of medical materials, which are usually solid materials and are used in bone regeneration, cardiology, or dermatology. Current advancements in science and technology provide several standard cytotoxicity testing methods that are sufficiently sensitive to detect various levels of cellular toxicity, i.e., from low to high. The aim was to compare the direct and indirect methodology described in the ISO guidelines UNE-EN ISO 10993-5:2009 Part 5. Methods: Cell proliferation was measured using WST-1 assay, and cytotoxicity was measured using LDH test kit. Results: The results indicate that the molecular surface of biomaterials have impact on the cytotoxicity and proliferation profile. Based on these results, we confirm that the indirect method does not provide a clear picture of the cell condition after the exposure to the surface, and moreover, cannot provide complete results about the effects of the material. Conclusions: Comparison of both methods shows that it is pivotal to investigate biomaterials at the very early stages using both indirect and direct methods to access the influence of the released toxins and surface of the material on the cell condition. MDPI 2022-10-25 /pmc/articles/PMC9657594/ /pubmed/36365516 http://dx.doi.org/10.3390/polym14214522 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chraniuk, Milena
Panasiuk, Mirosława
Hovhannisyan, Lilit
Żołędowska, Sabina
Nidzworski, Dawid
Ciołek, Lidia
Woźniak, Anna
Jaegermann, Zbigniew
Biernat, Monika
Gromadzka, Beata
The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title_full The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title_fullStr The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title_full_unstemmed The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title_short The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches
title_sort preliminary assessment of new biomaterials necessitates a comparison of direct and indirect cytotoxicity methodological approaches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657594/
https://www.ncbi.nlm.nih.gov/pubmed/36365516
http://dx.doi.org/10.3390/polym14214522
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